Cruz-Rodríguez Luis A, Chu Fu-Lin E
Virginia Institute of Marine Science, School of Marine Science, College of William and Mary, Department of Environmental Sciences, Box 1346, Gloucester Point, VA 23062-1346, USA.
Aquat Toxicol. 2002 Oct 30;60(3-4):157-68. doi: 10.1016/s0166-445x(02)00008-5.
Sediments are a potentially significant source of pollutants, containing not only organic contaminants but heavy metals as well. The heat shock protein response (HSP70 family) in the eastern oyster exposed to suspended clay particles spiked with polynuclear aromatic hydrocarbons (PAHs) and to suspended field contaminated sediments (SFCS) was investigated. In experiment 1, oysters were exposed to 1.0, 1.5 or 2.0 g suspended clay particles with concentrations of 65.6, 159.0 and 242 micro g PAHs per g of wet clay particles, respectively, and sampled after 40 days. Controls were exposed to 0, 1.0, 1.5, and 2.0 g suspended unspiked clay particles. In experiment 2, oysters were exposed to 0, 1.0, 1.5, and 2.0 g SFCS and the HSP70 expression was determined after 5, 10, 20 and 40 days exposure. Oysters exposed to suspended clay particles spiked with PAHs showed a significant increase in HSP70 levels, while oysters exposed to 1.0, 1.5 or 2.0 g suspended unspiked clay particles did not show changes (P=0.78) in HSP70 levels compared to the group receiving 0 g clay particles. Exposure to the SFCS resulted in a significant increase in HSP70 as a function of exposure (P<0.001) and treatment (P=0.006). The response, however, was not dose dependent. Compared to the control group (0 g SFCS), groups exposed to 1.0, 1.5 and 2.0 g SFCS reached significantly higher levels in HSP70 at 40 days of exposure, with those exposed to 2.0 g SFCS expressing the highest levels. The HSP70 expression for each treatment showed fluctuations at various time intervals. No mortalities were recorded during the exposure experiments. The major contaminants in the SFCS were PAHs, heavy metals and polychlorinated biphenyls (PCBs). These results reveal that exposure to PAHs sorbed to clay particles and to SFCS induced a HSP70 response in the eastern oyster.
沉积物是潜在的重要污染物来源，不仅含有有机污染物，还含有重金属。研究了暴露于添加多环芳烃（PAHs）的悬浮粘土颗粒以及暴露于现场受污染悬浮沉积物（SFCS）的东部牡蛎的热休克蛋白反应（HSP70家族）。在实验1中，牡蛎分别暴露于1.0、1.5或2.0克悬浮粘土颗粒，每克湿粘土颗粒中PAHs浓度分别为65.6、159.0和242微克，并在40天后取样。对照组暴露于0、1.0、1.5和2.0克未添加PAHs的悬浮粘土颗粒。在实验2中，牡蛎暴露于0、1.0、1.5和2.0克SFCS，并在暴露5、10、20和40天后测定HSP70表达。暴露于添加PAHs的悬浮粘土颗粒的牡蛎HSP70水平显著升高，而暴露于1.0、1.5或2.0克未添加PAHs的悬浮粘土颗粒的牡蛎与接受0克粘土颗粒的组相比，HSP70水平没有变化（P = 0.78）。暴露于SFCS导致HSP70随暴露时间（P < 0.001）和处理（P = 0.006）显著增加。然而，该反应不是剂量依赖性的。与对照组（0克SFCS）相比，暴露于1.0、1.5和2.0克SFCS组在暴露40天时HSP70水平显著更高，其中暴露于2.0克SFCS的组表达水平最高。每种处理的HSP70表达在不同时间间隔出现波动。暴露实验期间未记录到死亡情况。SFCS中的主要污染物是PAHs、重金属和多氯联苯（PCBs）。这些结果表明，暴露于吸附在粘土颗粒上的PAHs以及暴露于SFCS会诱导东部牡蛎产生HSP70反应。
