Levesque H M, Moon T W, Campbell P G C, Hontela A
Département des Sciences Biologiques, Toxen Research Center, Université du Québec à Montréal, C.P 8888, Succursale Centre-Ville, Québec, H3C 3P8, Montreal, Canada.
Aquat Toxicol. 2002 Oct 30;60(3-4):257-67. doi: 10.1016/s0166-445x(02)00012-7.
The effects of heavy metals on growth, intermediary metabolism and enzyme activities were investigated in yellow perch (Perca flavescens), sampled in summer and fall from lakes situated along a contamination gradient of Cd, Zn and Cu in the mining region of Rouyn-Noranda, Québec. An exposure-dependent decrease in condition factor was observed in both seasons. Liver glycogen and triglyceride reserves were higher in summer than in fall in fish from the reference lake, while the seasonal pattern was different in fish from the contaminated lakes. Plasma free fatty acids (FFA) levels were also influenced by season and contamination. Activities of malic enzyme (ME) and glucose 6-phosphate dehydrogenase (G6PDH) in the liver were higher in the summer than in the fall in reference lakes whereas no seasonal variations were detected in fish from contaminated lakes. Activities of pyruvate kinase (PyK), aspartate transaminase (AST), phosphoenolpyruvate carboxykinase (PEPCK) and malate dehydrogenase (MDH), were higher in fish from contaminated lakes in fall but not in summer. Chronic exposure of yellow perch to sublethal levels of heavy metals impairs growth and alters the seasonal cycling of liver glycogen and triglycerides as well as the activities of metabolic enzymes.
在魁北克省鲁安 - 诺兰达矿区，沿着镉、锌和铜污染梯度的湖泊中，于夏季和秋季采集了黄鲈（Perca flavescens）样本，研究了重金属对其生长、中间代谢和酶活性的影响。在两个季节中均观察到状况因子随暴露程度降低。来自对照湖泊的鱼类，肝脏糖原和甘油三酯储备在夏季高于秋季，而来自污染湖泊的鱼类其季节性模式则不同。血浆游离脂肪酸（FFA）水平也受季节和污染的影响。对照湖泊中，肝脏苹果酸酶（ME）和葡萄糖6 - 磷酸脱氢酶（G6PDH）的活性夏季高于秋季，而污染湖泊中的鱼类未检测到季节性变化。丙酮酸激酶（PyK）、天冬氨酸转氨酶（AST）、磷酸烯醇式丙酮酸羧激酶（PEPCK）和苹果酸脱氢酶（MDH）的活性，秋季时污染湖泊中的鱼类高于夏季，但夏季时未出现这种情况。黄鲈长期暴露于亚致死水平的重金属会损害其生长，并改变肝脏糖原和甘油三酯的季节性循环以及代谢酶的活性。
