Weston G C, Haviv I, Rogers P A W
Centre for Women's Health Research, Department of Obstetrics and Gynaecology, Monash University, 246 Clayton Rd, Clayton, Victoria 3168, Australia.
Mol Hum Reprod. 2002 Sep;8(9):855-63. doi: 10.1093/molehr/8.9.855.
There is evidence that the vasculature of different organs display different functional characteristics in response to cytokines and growth factors. The aim of this study was to use cDNA gene expression microarray to analyse changes in gene expression following stimulation of myometrial microvascular endothelial cells (MMECs) with vascular endothelial growth factor (VEGF). Primary isolates of MMECs were obtained from fresh hysterectomy specimens and purified with magnetic beads. Cells were stimulated with 15 ng/ml VEGF for 3, 6 and 12 h, and two unstimulated experiments served as controls. A total of six arrays was performed over these time-points. A total of 110 genes were identified as up-regulated by VEGF, 19% of which (21 genes) have previously been reported as up-regulated by VEGF or by angiogenesis. Among the novel genes to be up-regulated by VEGF were brain-derived growth factor, oxytocin receptor and estrogen sulphotransferase. The significance of the genes identified in the physiological and pathological functioning of the myometrial vasculature is discussed.
有证据表明，不同器官的脉管系统在对细胞因子和生长因子的反应中表现出不同的功能特征。本研究的目的是使用cDNA基因表达微阵列分析血管内皮生长因子（VEGF）刺激子宫肌层微血管内皮细胞（MMECs）后基因表达的变化。MMECs的原代分离物从新鲜子宫切除标本中获得，并用磁珠纯化。细胞用15 ng/ml VEGF刺激3、6和12小时，两个未刺激的实验作为对照。在这些时间点总共进行了六个阵列实验。总共110个基因被鉴定为被VEGF上调，其中19%（21个基因）先前已被报道被VEGF或血管生成上调。被VEGF上调的新基因包括脑源性生长因子、催产素受体和雌激素磺基转移酶。讨论了所鉴定基因在子宫肌层脉管系统生理和病理功能中的意义。
