pH值的变化可引发太河单睾吸虫后尾蚴的脱囊。

Excystation of Haplorchis taichui metacercariae could be elicited by change in pH.

作者信息

Ooi Hong Kean, Chen Ching-I, Oku Yuzaburo

机构信息

Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan.

出版信息

Jpn J Vet Res. 2002 May;50(1):3-7.

PMID:12201017

Abstract

The effect of different component of the intestinal fluid on the excystation of Haplorchis taichui metacercariae was examined in vitro. Encysted metacercariae obtained by pepsin digestion of fish muscle showed that the highest percentage of metacercariae excystation could be obtained after being treated with trypsin at pH 7.5. However, it was also observed that excystation of the metacercariae could also be effected by changing the pH to the basic condition. The most optimum pH for the metacercariae excystation was found to be 7.5. This method of obtaining the excysted metacercariae could prove to be useful for taxonomical study of trematode in general.

摘要

体外研究了肠液不同成分对太河单睾吸虫后尾蚴脱囊的影响。通过胃蛋白酶消化鱼肌肉获得的包囊后尾蚴表明，在pH 7.5条件下用胰蛋白酶处理后，后尾蚴脱囊的百分比最高。然而，还观察到，将pH值改变为碱性条件也会影响后尾蚴的脱囊。发现后尾蚴脱囊的最适宜pH值为7.5。这种获得脱囊后尾蚴的方法可能对一般吸虫的分类学研究有用。