Vyas H K, Izco J M, Jiménez-Flores R
Dairy Products Technology Center, California Polytechnic State University, San Luis Obispo 93407, USA.
J Dairy Sci. 2002 Jul;85(7):1639-45. doi: 10.3168/jds.S0022-0302(02)74236-7.
Affinity separation of beta-lactoglobulin in its native form with all-trans-retinal immobilized on calcium bio-silicate was scaled up and applied to separate it from industrial sweet whey. Three different methods of mixing the modified calcium bio-silicate and whey for the interaction between all-trans-retinal and beta-lactoglobulin were tried at pilot scale. The three methods used were 1) a column packed with calcium bio-silicate, 2) a stirred tank, and 3) a fluidized bed column of calcium bio-silicate particles. Adsorption and desorption of beta-lactoglobulin were carried out at pH 5.1 and 7.0, using 0.01 and 0.1 M phosphate buffers, respectively. The phosphate buffer containing desorbed beta-lactoglobulin was concentrated 20 times using ultrafiltration and then freeze-dried. The packed column, stirred tank, and fluidized bed column produced beta-lactoglobulin with purity of 80, >95, and >95%, and recovery of 0.65, 2.88, and 2.88 g per kilogram of calcium bio-silicate, respectively. The comparative poor purity and recovery of beta-lactoglobulin in the case of the packed column was attributed to insufficient contact between the passing fluids and the calcium bio-silicate during adsorption, desorption, and intermittent washing. The fluidized bed column method, with a gentle mixing action, was considered the best suited for further scale up to the industrial level.
将固定有全反式视黄醛的天然形式的β-乳球蛋白在钙生物硅酸盐上进行亲和分离，并将其放大应用于从工业甜乳清中分离β-乳球蛋白。在中试规模下尝试了三种不同的将改性钙生物硅酸盐和乳清混合以实现全反式视黄醛与β-乳球蛋白之间相互作用的方法。所使用的三种方法分别是：1）填充有钙生物硅酸盐的柱；2）搅拌槽；3）钙生物硅酸盐颗粒的流化床柱。β-乳球蛋白的吸附和解吸分别在pH 5.1和7.0下进行，分别使用0.01和0.1 M的磷酸盐缓冲液。含有解吸的β-乳球蛋白的磷酸盐缓冲液通过超滤浓缩20倍，然后冷冻干燥。填充柱、搅拌槽和流化床柱产生的β-乳球蛋白纯度分别为80%、>95%和>95%，每千克钙生物硅酸盐的回收率分别为0.65、2.88和2.88 g。填充柱中β-乳球蛋白的纯度和回收率相对较差，这归因于在吸附、解吸和间歇洗涤过程中通过的流体与钙生物硅酸盐之间的接触不足。具有温和混合作用的流化床柱方法被认为最适合进一步扩大到工业规模。
