Linez-Bataillon P, Monchau F, Bigerelle M, Hildebrand H F
Groupe de Recherche sur les Biomatériaux, UPRES EA 1049, Faculté de Médecine, F-59045 Lille Cedex, France.
Biomol Eng. 2002 Aug;19(2-6):133-41. doi: 10.1016/s1389-0344(02)00024-2.
This work investigates the role of the surface roughness of Ti6Al4V on the cell morphology, proliferation and adhesion, and in particular on the variation of the expression of cell adhesion proteins. Standardised test samples with five different surface preparations are used: sandblasted, 80, 1200, and 4000 grade polished, mirror polished. Surface roughness is analysed by Scanning Electron Microscopy and LASER Confocal Microscopy. Cell culture experiments are performed with MC3T3-E1 mouse osteoblasts after 3 days culture: proliferation rate, morphology and adhesion are assessed. The variations of expression of cell adhesion proteins are evidenced by indirect immune fluorescence method: actin from the cytoskeleton, vinculin from the focal adhesion complex, fibronectin and collagen I from the extracellular matrix. The results reveal a clear influence of surface roughness of Ti6Al4V on cell proliferation, morphology and adhesion. A significant correlation is established between surface roughness and cell growth. More the surface is smooth more the osteoblasts proliferate and appear spread out on the test samples. In addition, the expression of adhesion proteins varies with respect to the surface roughness. These results indicate a direct relationship between the decrease of cell adhesion and the increase of cell proliferation on mirror polished materials.
本研究探讨了Ti6Al4V表面粗糙度对细胞形态、增殖和黏附的作用，特别是对细胞黏附蛋白表达变化的影响。使用了具有五种不同表面处理的标准化测试样品：喷砂处理、80级、1200级和4000级抛光、镜面抛光。通过扫描电子显微镜和激光共聚焦显微镜分析表面粗糙度。在培养3天后，用MC3T3-E1小鼠成骨细胞进行细胞培养实验：评估增殖率、形态和黏附情况。通过间接免疫荧光法证明细胞黏附蛋白表达的变化：细胞骨架中的肌动蛋白、黏着斑复合物中的纽蛋白、细胞外基质中的纤连蛋白和I型胶原蛋白。结果显示Ti6Al4V表面粗糙度对细胞增殖、形态和黏附有明显影响。表面粗糙度与细胞生长之间建立了显著相关性。表面越光滑，成骨细胞在测试样品上的增殖和铺展就越明显。此外，黏附蛋白的表达随表面粗糙度而变化。这些结果表明，在镜面抛光材料上，细胞黏附的减少与细胞增殖的增加之间存在直接关系。
