Department of Anatomy and Cell Biology, Institute of Cancer Research and Translational Medicine, Medical Research Center, University of Oulu, P.O. Box 5000, 90014 Oulu, Finland.
Department of Chemistry, University of Helsinki, P.O. Box 55, 00014 Helsinki, Finland.
Biomolecules. 2022 Apr 29;12(5):654. doi: 10.3390/biom12050654.
The increasing demand for bone implants with improved osseointegration properties has prompted researchers to develop various coating types for metal implants. Atomic layer deposition (ALD) is a method for producing nanoscale coatings conformally on complex three-dimensional surfaces. We have prepared hydroxyapatite (HA) coating on titanium (Ti) substrate with the ALD method and analyzed the biocompatibility of this coating in terms of cell adhesion and viability.
HA coatings were prepared on Ti substrates by depositing CaCO films by ALD and converting them to HA by wet treatment in dilute phosphate solution. MC3T3-E1 preosteoblasts were cultured on ALD-HA, glass slides and bovine bone slices. ALD-HA and glass slides were either coated or non-coated with fibronectin. After 48h culture, cells were imaged with scanning electron microscopy (SEM) and analyzed by vinculin antibody staining for focal adhesion localization. An 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) test was performed to study cell viability.
Vinculin staining revealed similar focal adhesion-like structures on ALD-HA as on glass slides and bone, albeit on ALD-HA and bone the structures were thinner compared to glass slides. This might be due to thin and broad focal adhesions on complex three-dimensional surfaces of ALD-HA and bone. The MTT test showed comparable cell viability on ALD-HA, glass slides and bone.
ALD-HA coating was shown to be biocompatible in regard to cell adhesion and viability. This leads to new opportunities in developing improved implant coatings for better osseointegration and implant survival.
对具有改善骨整合性能的骨植入物的需求不断增加，促使研究人员开发出各种用于金属植入物的涂层类型。原子层沉积（ALD）是在复杂三维表面上制备纳米级涂层的方法。我们已经使用 ALD 方法在钛（Ti）基底上制备了羟基磷灰石（HA）涂层，并分析了该涂层在细胞黏附和活力方面的生物相容性。
通过 ALD 沉积 CaCO 薄膜，并在稀磷酸盐溶液中进行湿处理将其转化为 HA，在 Ti 基底上制备 HA 涂层。将 MC3T3-E1 前成骨细胞培养在 ALD-HA、载玻片和牛骨片上。ALD-HA 和载玻片要么涂覆有纤连蛋白，要么没有涂覆。培养 48 小时后，用扫描电子显微镜（SEM）对细胞进行成像，并通过黏着斑蛋白抗体染色分析黏附斑的定位。通过 3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）试验研究细胞活力。
黏着斑蛋白染色显示，ALD-HA 上的黏附斑样结构与载玻片和骨上的相似，尽管 ALD-HA 和骨上的结构比载玻片上的更薄。这可能是由于 ALD-HA 和骨的复杂三维表面上的黏附斑较薄且较宽。MTT 试验表明，ALD-HA、载玻片和骨上的细胞活力相当。
ALD-HA 涂层在细胞黏附和活力方面表现出良好的生物相容性。这为开发更好的骨整合和植入物存活的改进型植入物涂层提供了新的机会。
