嗜酸性红假单胞菌单个光捕获1复合体的光谱学研究。
Spectroscopy on individual light-harvesting 1 complexes of Rhodopseudomonas acidophila.
作者信息
Ketelaars Martijn, Hofmann Clemens, Köhler Jürgen, Howard Tina D, Cogdell Richard J, Schmidt Jan, Aartsma Thijs J
机构信息
Department of Biophysics, Huygens Laboratory, Leiden University, 2300 RA Leiden, The Netherlands.
出版信息
Biophys J. 2002 Sep;83(3):1701-15. doi: 10.1016/S0006-3495(02)73938-6.
Abstract
In this paper the fluorescence-excitation spectra of individual LH1-RC complexes (Rhodopseudomonas acidophila) at 1.2 K are presented. All spectra show a limited number of broad bands with a characteristic polarization behavior, indicating that the excitations are delocalized over a large number of pigments. A significant variation in the number of bands, their bandwidths, and polarization behavior is observed. Only 30% of the spectra carry a clear signature of delocalized excited states of a circular structure of the pigments. The large spectral variety suggests that besides site heterogeneity also structural heterogeneity determines the optical spectrum of the individual LH1-RC complexes. Further research should reveal if such heterogeneity is a native property of the complex or induced during the experimental procedures.
摘要
本文展示了嗜酸红假单胞菌单个LH1-RC复合物在1.2 K时的荧光激发光谱。所有光谱均显示出数量有限的宽带，具有特征性的偏振行为，这表明激发在大量色素上是离域的。观察到谱带数量、带宽和偏振行为存在显著变化。只有30%的光谱带有色素圆形结构离域激发态的清晰特征。巨大的光谱多样性表明，除了位点异质性外，结构异质性也决定了单个LH1-RC复合物的光谱。进一步的研究应揭示这种异质性是复合物的固有特性还是在实验过程中诱导产生的。
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