Fraser N J, Dominy P J, Ucker B, Simonin I, Scheer H, Cogdell R J
Division of Biochemistry and Molecular Biology, IBLS, University of Glasgow, United Kingdom.
Biochemistry. 1999 Jul 27;38(30):9684-92. doi: 10.1021/bi990796i.
A method is described which allows the selective release and removal of the Bchla-B800 molecules from the LH2 complex of Rhodopseudomonas acidophila 10050. This procedure also allows reconstitution of approximately 80% of the empty binding sites with native Bchla. As shown by circular dichroism spectroscopy, the overall structures of the B850-only and reconstituted complexes are not affected by the pigment-exchange procedure. The pigments reconstituted into the B800 sites can also efficiently transfer excitation energy to the Bchla-B850 molecules.
本文描述了一种方法，该方法可从嗜酸红假单胞菌10050的LH2复合物中选择性释放和去除Bchla - B800分子。此过程还能使约80%的空结合位点用天然Bchla进行重构。圆二色光谱显示，仅含B850的复合物和重构复合物的整体结构不受色素交换过程的影响。重构到B800位点的色素也能有效地将激发能转移到Bchla - B850分子上。
