Lamb David J, Avades Tony Y, Allen Michael D, Anwar Khurshid, Kass George E N, Ferns Gordon A A
Centre for Clinical Science and Measurement, School of Biomedical and Life Sciences, University of Surrey, Surrey GU27XH, Guildford, UK
Atherosclerosis. 2002 Oct;164(2):229-36. doi: 10.1016/s0021-9150(02)00068-0.
We have previously shown that dietary copper supplementation modulates the formation of atherosclerotic lesions in the cholesterol-fed rabbit. In the present study, we have investigated the effects of copper supplementation on the cellular composition and characteristics of atherosclerotic lesions in cholesterol-fed NZW rabbits. Rabbits received a 1% cholesterol diet with or without 0.02% copper acetate (containing 12 and 0.3 mg copper per 100 g diet, respectively) for 12 weeks. The tunica intima was significantly smaller in the animals receiving copper supplements (0.016+/-0.005 vs. 0.068+/-0.019 mm(2), P<0.05) and this was accompanied by a significant increase in aortic copper content (4.0+/-0.8 vs. 1.8+/-0.2 microg/g tissue, P<0.05). The percentage area staining for smooth muscle cells (HHF-35 positive) was significantly lower in the intima of animals receiving copper supplements (7.13+/-1.02 vs. 9.72+/-0.82%, P<0.05). However, there were no significant differences in area staining for macrophages (RAM-11 positive) between groups (22.6+/-7.9 vs. 23.3+/-4.9%). There were also significantly fewer apoptotic cells (0.96+/-0.33 vs. 2.54+/-0.56%, P<0.005) in the aortic intima from animals supplemented with copper, but no difference in the number of proliferating cells. However, there was a reduction in intimal collagen staining (Sirius red positivity) in the animals receiving a copper supplement. Taken together, these data show that dietary copper can significantly affect the composition and progression of atherosclerotic lesions.
我们之前已经表明，膳食补充铜可调节喂食胆固醇的兔子动脉粥样硬化病变的形成。在本研究中，我们研究了补充铜对喂食胆固醇的新西兰白兔动脉粥样硬化病变的细胞组成和特征的影响。兔子接受含1%胆固醇的饮食，添加或不添加0.02%醋酸铜（分别含每100克饮食12毫克和0.3毫克铜），持续12周。接受铜补充剂的动物的内膜明显更小（0.016±0.005对0.068±0.019平方毫米，P<0.05），同时主动脉铜含量显著增加（4.0±0.8对1.8±0.2微克/克组织，P<0.05）。接受铜补充剂的动物内膜中平滑肌细胞（HHF-35阳性）的染色面积百分比显著降低（7.13±1.02对9.72±0.82%，P<0.05）。然而，各组之间巨噬细胞（RAM-11阳性）的染色面积没有显著差异（22.6±7.9对23.3±4.9%）。补充铜的动物主动脉内膜中的凋亡细胞也显著减少（0.96±0.33对2.54±0.56%，P<0.005），但增殖细胞数量没有差异。然而，接受铜补充剂的动物内膜胶原染色（天狼星红阳性）减少。综上所述，这些数据表明膳食铜可显著影响动脉粥样硬化病变的组成和进展。
