Sigurdsson Stefan, Van Komen Stephen, Petukhova Galina, Sung Patrick
Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78254-3207, USA.
J Biol Chem. 2002 Nov 8;277(45):42790-4. doi: 10.1074/jbc.M208004200. Epub 2002 Aug 29.
Human Rad51 (hRad51) and Rad54 proteins are key members of the RAD52 group required for homologous recombination. We show an ability of hRad54 to promote transient separation of the strands in duplex DNA via its ATP hydrolysis-driven DNA supercoiling function. The ATPase, DNA supercoiling, and DNA strand opening activities of hRad54 are greatly stimulated through an interaction with hRad51. Importantly, we demonstrate that hRad51 and hRad54 functionally cooperate in the homologous DNA pairing reaction that forms recombination DNA intermediates. Our results should provide a biochemical model for dissecting the role of hRad51 and hRad54 in recombination reactions in human cells.
人类Rad51（hRad51）蛋白和Rad54蛋白是同源重组所需的RAD52蛋白家族的关键成员。我们发现hRad54具有通过其ATP水解驱动的DNA超螺旋功能促进双链DNA中链的瞬时分离的能力。hRad54的ATP酶、DNA超螺旋和DNA链打开活性通过与hRad51的相互作用而得到极大刺激。重要的是，我们证明hRad51和hRad54在形成重组DNA中间体的同源DNA配对反应中发挥功能协同作用。我们的结果应为剖析hRad51和hRad54在人类细胞重组反应中的作用提供一个生化模型。
