胡萝卜（Daucus carrota L.）中果胶甲酯酶的部分纯化、特性鉴定以及热失活和高压失活研究

Partial purification, characterization, and thermal and high-pressure inactivation of pectin methylesterase from carrots (Daucus carrota L.).

作者信息

Ly-Nguyen Binh, Van Loey Ann M, Fachin Diana, Verlent Isabel, Indrawati Indrawati, Hendrickx Marc E

机构信息

Laboratory of Food Technology, Department of Food and Microbial Technology, Faculty of Agricultural and Applied Biological Sciences, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium.

出版信息

J Agric Food Chem. 2002 Sep 11;50(19):5437-44. doi: 10.1021/jf011666v.

DOI:10.1021/jf011666v

PMID:12207488

Abstract

Pectin methylesterase (PME) from carrots (Daucus carrota L.) was extracted and purified by affinity chromatography on a CNBr-Sepharose 4B-PME inhibitor column. A single protein and PME activity peak was obtained. A biochemical characterization in terms of molar mass (MM), isoelectric points (pI), and kinetic parameters of carrot PME was performed. In a second step, the thermal and high-pressure stability of the enzyme was studied. Isothermal and combined isothermal-isobaric inactivation of purified carrot PME could be described by a fractional-conversion model.

摘要

通过在溴化氰-琼脂糖4B-果胶甲酯酶（PME）抑制剂柱上进行亲和层析，从胡萝卜（Daucus carrota L.）中提取并纯化了果胶甲酯酶（PME）。获得了一个单一的蛋白质和PME活性峰。对胡萝卜PME的摩尔质量（MM）、等电点（pI）和动力学参数进行了生化表征。在第二步中，研究了该酶的热稳定性和高压稳定性。纯化后的胡萝卜PME的等温失活和等温-等压联合失活可用分数转化率模型来描述。