HLA - DQ8以及HLA - DQ8 - DR4单倍型与乳胶过敏医护人员中橡胶素特异性IgE免疫反应呈正相关。

HLA-DQ8 and the HLA-DQ8-DR4 haplotype are positively associated with the hevein-specific IgE immune response in health care workers with latex allergy.

作者信息

Rihs Hans-Peter, Chen Zhiping, Ruëff Franziska, Cremer Reinhold, Raulf-Heimsoth Monika, Baur Xaver, Moneret-Vautrin Denise Anne, Brüning Thomas

机构信息

Research Institute for Occupational Medicine of the Berufsgenossenschaften (BGFA), Institute of the Ruhr-University Bochum, Germany.

出版信息

J Allergy Clin Immunol. 2002 Sep;110(3):507-14. doi: 10.1067/mai.2002.127282.

DOI:10.1067/mai.2002.127282

PMID:12209103

Abstract

BACKGROUND

Hevein is one of the most important latex allergens affecting health care workers (HCWs).

OBJECTIVE

Because the genetically determined susceptibility is one important factor regulating type I allergy, the association between the hevein-specific IgE immune response and HLA class II alleles of DQB1 and DRB1, DRB3, DRB4, and DRB5 was studied.

METHODS

The distribution of HLA-DQB1 and DRB1, DRB3, DRB4, and DRB5 in 269 HCWs with latex allergy, 56 latex-sensitized patients with spina bifida (SB), and 90 nonatopic control subjects under special consideration for hevein-specific IgE was examined.

RESULTS

Seventy percent (189/269) of the HCWs with latex allergy and 39% (22/56) of the latex-sensitized patients with SB had increased hevein-specific IgE antibody concentrations (>0.35 kU/L). HLA data analysis revealed significantly increased phenotype frequencies for DQB10302 (DQ8; 91/189 [48%]) and DRB104 (DR4; 102/189 [54%]) in hevein-positive HCWs with latex allergy compared with the 80 hevein-negative HCWs with latex allergy (DQB10302: 16/80 [20%], corrected P value [P (c)] = 7.1 x 10(-4); DRB104: 23/80 [29%], P (c) =.01) and with control subjects (DQB10302: 16/89 [18%], P (c) = 1 x 10(-4); DRB104: 22/90 [24%], P (c) = 3.2 x 10(-4)). The DQ8-DR4 haplotype frequency was significantly elevated in HCWs with hevein-specific IgE antibodies when compared with that in HCWs without hevein-specific IgE antibodies (47% vs 18%, P (c) = 5.3 x 10(-4)) or control subjects (47% vs 18%, P (c) = 9.6 x 10(-4)). In contrast, latex-sensitized patients with SB with hevein-specific IgE antibodies showed an increased but not significant DQB1*0302 frequency (7/22 [32%] vs 2/34 [6%], P =.02, P (c) = not significant) compared with that seen in those without hevein-specific IgE antibodies.

CONCLUSION

The DQB10302 (DQ8) alone, the DQB10302 (DQ8)-DRB1*04 (DR4) haplotype, or both are significantly involved in the hevein-specific IgE immune response in HCWs with latex allergy.

摘要

背景

橡胶素是影响医护人员（HCW）健康的最重要的乳胶过敏原之一。

目的

由于基因决定的易感性是调节I型过敏的一个重要因素，因此研究了橡胶素特异性IgE免疫反应与DQB1以及DRB1、DRB3、DRB4和DRB5的HLA II类等位基因之间的关联。

方法

在特别考虑橡胶素特异性IgE的情况下，检测了269名乳胶过敏的医护人员、56名脊柱裂（SB）乳胶致敏患者和90名非特应性对照受试者中HLA-DQB1以及DRB1、DRB3、DRB4和DRB5的分布情况。

结果

70%（189/269）的乳胶过敏医护人员和39%（22/56）的SB乳胶致敏患者的橡胶素特异性IgE抗体浓度升高（>0.35 kU/L）。HLA数据分析显示，与80名橡胶素阴性的乳胶过敏医护人员相比，橡胶素阳性的乳胶过敏医护人员中DQB10302（DQ8；91/189 [48%]）和DRB104（DR4；102/189 [54%]）的表型频率显著升高（DQB10302：16/80 [20%]，校正P值[P（c）]=7.1×10⁻⁴；DRB104：23/80 [29%]，P（c）=.01），与对照受试者相比也是如此（DQB10302：16/89 [18%]，P（c）=1×10⁻⁴；DRB104：22/90 [24%]，P（c）=3.2×10⁻⁴）。与没有橡胶素特异性IgE抗体的医护人员相比，有橡胶素特异性IgE抗体的医护人员中DQ8-DR4单倍型频率显著升高（47%对18%，P（c）=5.3×10⁻⁴），与对照受试者相比也是如此（47%对18%，P（c）=9.6×10⁻⁴）。相比之下，与没有橡胶素特异性IgE抗体的SB乳胶致敏患者相比，有橡胶素特异性IgE抗体的SB乳胶致敏患者的DQB1*0302频率有所升高，但差异不显著（7/22 [32%]对2/34 [6%]，P =.02，P（c）=不显著）。