鉴定橡胶树胶乳中的橡胶素（Hev b 6.02）为乳胶过敏患者中与鳄梨果实发生主要交叉反应的变应原。
Identification of hevein (Hev b 6.02) in Hevea latex as a major cross-reacting allergen with avocado fruit in patients with latex allergy.
作者信息
Chen Z, Posch A, Cremer R, Raulf-Heimsoth M, Baur X
机构信息
Research Institute for Occupational Medicine (BGFA), Ruhr-University of Bochum, Germany.
出版信息
J Allergy Clin Immunol. 1998 Sep;102(3):476-81. doi: 10.1016/s0091-6749(98)70138-1.
BACKGROUND
Recent studies demonstrated that allergy to natural rubber latex is frequently associated with hypersensitivity to avocado fruit. The responsible cross-sensitizing allergen has not been identified.
OBJECTIVE
The purpose of this study was to investigate the cross-reactivity of a latex major allergen, hevein, with avocado proteins.
METHODS
Serum samples from 118 health care workers (HCWs) allergic to latex (HCW group) and 78 patients with spina bifida (SB) allergic to latex (SB group) were included in this study. Anti-hevein and anti-avocado IgE antibodies were measured by enzyme-linked allergosorbent assay. Cross-reactivity of hevein to avocado proteins was assessed by inhibition of the IgE binding in individual patients' sera containing IgE antibodies to both hevein and avocado.
RESULTS
The prevalence of seropositive IgE antibodies to avocado was found to be strongly associated with the presence of hevein-specific IgE antibodies in subjects of both groups (P < .001). Sixty-seven of 91 (73%) subjects from the HCW group and all 19 subjects in the SB group with positive IgE antibodies to hevein also had elevated IgE values to avocado. Competitive RAST inhibition with 42 sera showed that IgE binding to avocado could be completely inhibited in 27 (64%) sera by preincubation with hevein. By contrast, the degrees of inhibition of IgE to hevein by avocado extract ranged from 0% to 36% (n = 16). These results indicate that sensitization to avocado in most patients allergic to latex is caused exclusively by IgE-binding epitopes present in hevein. Results of immunoblots and immunoblot inhibition with 11 serum samples confirmed that a 30-kd protein in avocado was the major IgE-binding component; the IgE-binding reactivity to this protein could be inhibited by hevein in all sera tested.
CONCLUSION
Hevein is the major cross-reacting allergen with avocado in subjects with latex allergy.
背景
最近的研究表明，对天然橡胶乳胶过敏常常与对鳄梨过敏相关。尚未确定起交叉致敏作用的过敏原。
目的
本研究旨在调查乳胶主要过敏原橡胶素与鳄梨蛋白的交叉反应性。
方法
本研究纳入了118名对乳胶过敏的医护人员（医护人员组）和78名对乳胶过敏的脊柱裂患者（脊柱裂组）的血清样本。通过酶联免疫吸附测定法检测抗橡胶素和抗鳄梨IgE抗体。通过抑制含有针对橡胶素和鳄梨的IgE抗体的个体患者血清中的IgE结合来评估橡胶素与鳄梨蛋白的交叉反应性。
结果
发现两组受试者中，鳄梨血清阳性IgE抗体的患病率与橡胶素特异性IgE抗体的存在密切相关（P <.001）。医护人员组91名受试者中有67名（73%）以及脊柱裂组所有19名对橡胶素有阳性IgE抗体的受试者对鳄梨的IgE值也升高。对42份血清进行的竞争性放射变应原吸附试验抑制显示，通过与橡胶素预孵育，27份（64%）血清中IgE与鳄梨的结合可被完全抑制。相比之下,鳄梨提取物对IgE与橡胶素的抑制程度为0%至36%（n = 16）。这些结果表明，大多数对乳胶过敏的患者对鳄梨的致敏完全是由橡胶素中存在的IgE结合表位引起的。用11份血清样本进行的免疫印迹和免疫印迹抑制结果证实，鳄梨中的一种30kd蛋白是主要的IgE结合成分；在所有检测的血清中，橡胶素均可抑制对该蛋白的IgE结合反应性。
结论
在乳胶过敏患者中，橡胶素是与鳄梨交叉反应的主要过敏原。
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