Trusova M Yu, Gladyshev M I
Institute of Biophysics of Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russia.
Microb Ecol. 2002 Oct;44(3):252-9. doi: 10.1007/s00248-002-2020-1. Epub 2002 Sep 6.
Using 16S rRNA gene sequence analyses we investigated the bacterial diversity of winter bacterioplankton of two eutrophic Siberian reservoirs. These reservoirs show similarity in phytoplankton community composition in spring and autumn but tend to differ in summer in exhibiting cyanobacterial bloom. Forty-eight unique partial 16S RNA gene sequences retrieved from two libraries were mostly affiliated with the class Actinobacteria, b subdivision of the class Proteobacteria, and the phylum Cytophaga-Flavobacterium-Bacteroides. The clone library of the pond exhibiting summer cyanobacterial bloom showed more diversity in sequence composition. A significant number of bacterial 16S rRNA gene clones were closely related to freshwater bacteria previously found in different aquatic ecosystems. This finding confirms the assumption that some bacterial clades are globally distributed.
利用16S rRNA基因序列分析，我们研究了两个富营养化的西伯利亚水库冬季浮游细菌的细菌多样性。这些水库在春季和秋季的浮游植物群落组成上具有相似性，但在夏季往往因出现蓝藻水华而有所不同。从两个文库中检索到的48个独特的部分16S RNA基因序列大多隶属于放线菌纲、变形菌纲β亚纲以及噬纤维菌-黄杆菌-拟杆菌门。呈现夏季蓝藻水华的池塘的克隆文库在序列组成上显示出更多样性。大量细菌16S rRNA基因克隆与先前在不同水生生态系统中发现的淡水细菌密切相关。这一发现证实了某些细菌类群在全球分布的假设。
