Reuss Frank U, Berdel Bianca, Heber Ricarda, Bantel-Schaal Ursula
Deutsches Krebsforschungszentrum, Angewandte Tumorvirologie F0400, Heidelberg, Germany.
J Med Virol. 2002 Oct;68(2):278-84. doi: 10.1002/jmv.10202.
Amphotropic murine leukemia virus (MLV) replicates in cells from various mammalian species including humans and is a potential contaminant in MLV vector preparations for human gene transfer studies. In general, MLV replication depends on the expression of viral genes under the control of 75 bp enhancer elements in the long terminal repeat. However, in specific human fibrosarcoma and lymphoma lines replication of amphotropic MLV is possible without these enhancers. Fibrosarcomas are malignant tumors of fibroblast origin. To test the replication potential of intact and enhancerless amphotropic MLV in untransformed cells, infection studies with these viruses were carried out in three types of primary human fibroblasts. Replication of amphotropic MLV is observed in two of three tested fibroblast strains. None of these primary human fibroblasts is permissive for enhancer-deficient MLV, suggesting that replication of this virus may be limited to transformed cells.
双嗜性鼠白血病病毒（MLV）可在包括人类在内的各种哺乳动物细胞中复制，并且在用于人类基因转移研究的MLV载体制剂中是一种潜在污染物。一般来说，MLV的复制依赖于长末端重复序列中75 bp增强子元件控制下的病毒基因表达。然而，在特定的人纤维肉瘤和淋巴瘤细胞系中，没有这些增强子，双嗜性MLV也可能复制。纤维肉瘤是起源于成纤维细胞的恶性肿瘤。为了测试完整的和无增强子的双嗜性MLV在未转化细胞中的复制潜力，用这些病毒在三种原代人成纤维细胞中进行了感染研究。在三种测试的成纤维细胞菌株中的两种中观察到双嗜性MLV的复制。这些原代人成纤维细胞中没有一种对缺乏增强子的MLV敏感，这表明这种病毒的复制可能仅限于转化细胞。
