Sverko Visnja, Balog Tihomir, Sobocanec Sandra, Gavella Mirjana, Marotti Tatjana
Department of Molecular Medicine, Rudjer Bosković Institute, Bijenicka 54, 10000 Zagreb, Croatia.
Exp Gerontol. 2002 Aug-Sep;37(8-9):1031-9. doi: 10.1016/s0531-5565(02)00083-9.
Oxidative modification of lipids, proteins and DNA by reactive oxygen species in the organism and imbalance between the concentrations of free radicals and the antioxidant defenses may be related to processes such as aging and diseases (cardiovascular, neurodegenerative, cancer, etc.). Although the relationship between oxidant status and antioxidant defence in aging of different species, organs or sexes has been investigated extensively, the studies have produced conflicting results. In order to determine the extent of age-associated alteration, oxidant production and antioxidant status were measured in tissues of CBA and AKR mice of both sexes. At the same time we will focus on lipid peroxidation (LPO) process and superoxide dismutase activity (SOD) of AKR mice related to ontogeny of thymic lymphoma in mice of different age and sex. Male and female CBA and AKR mice aged 3, 6, 12 or 18 months were used. Lipid-bound sialic acid (LSA) content was determined as a malignancy marker. LPO processes of CBA and AKR mice were monitored according to the presence of thiobarbituric acid reactive substances (TBARS) in liver and thymus, and antioxidant status as SOD activity in whole blood. TBARS concentration increased significantly with age in the liver of CBA and AKR mice of both sexes, but only in male thymuses of both strains. TBARS concentration in female thymuses of both strains was unchanged during aging. Thus, age-associated LPO processes of tumor-free mice of both strains were tissue-dependent. In the liver of tumor-bearing CBA and AKR mice as well as in thymuses of AKR mice, TBARS concentration significantly decreased and was neither sex nor tissue related. SOD activity was strain-dependent but independent of sex. However, SOD activity in mice with developed thymomas was drastically reduced in comparison to tumor-free mice. Our data indicate that age associated LPO processes in both strains are only tissue-dependent and SOD activity mainly strain-dependent in tumor-free mice. In tumor-bearing mice LPO processes and SOD activity were not tissue, sex or strain dependent.
生物体内活性氧对脂质、蛋白质和DNA的氧化修饰以及自由基浓度与抗氧化防御之间的失衡可能与衰老和疾病（心血管疾病、神经退行性疾病、癌症等）等过程有关。尽管不同物种、器官或性别的衰老过程中氧化状态与抗氧化防御之间的关系已得到广泛研究，但研究结果相互矛盾。为了确定与年龄相关的变化程度，我们检测了雄性和雌性CBA和AKR小鼠组织中的氧化剂生成和抗氧化状态。同时，我们将重点关注不同年龄和性别的AKR小鼠与胸腺淋巴瘤个体发育相关的脂质过氧化（LPO）过程和超氧化物歧化酶活性（SOD）。使用了3、6、12或18月龄的雄性和雌性CBA和AKR小鼠。测定脂质结合唾液酸（LSA）含量作为恶性肿瘤标志物。根据肝脏和胸腺中硫代巴比妥酸反应性物质（TBARS）的存在情况监测CBA和AKR小鼠的LPO过程，并将全血中SOD活性作为抗氧化状态。在雄性和雌性CBA和AKR小鼠的肝脏中，TBARS浓度随年龄显著增加，但仅在两个品系的雄性胸腺中增加。两个品系雌性胸腺中的TBARS浓度在衰老过程中没有变化。因此，两个品系无肿瘤小鼠与年龄相关的LPO过程具有组织依赖性。在荷瘤CBA和AKR小鼠的肝脏以及AKR小鼠的胸腺中，TBARS浓度显著降低，且与性别和组织无关。SOD活性具有品系依赖性，但与性别无关。然而，与无肿瘤小鼠相比，患有胸腺瘤的小鼠的SOD活性大幅降低。我们的数据表明，在无肿瘤小鼠中，两个品系与年龄相关的LPO过程仅具有组织依赖性，而SOD活性主要具有品系依赖性。在荷瘤小鼠中，LPO过程和SOD活性与组织、性别或品系无关。
