Carré Jean-Luc, Goetz Brian D, O'Connor Lawrence T, Bremer Quentin, Duncan Ian D
Service de Biochimie et Biologie Moléculaire, Faculté de Médecine, 22 Avenue C. Desmoulins, 29200 Brest, France.
Neurosci Lett. 2002 Sep 13;330(1):17-20. doi: 10.1016/s0304-3940(02)00709-7.
The Long Evans shaker (les) rat is a myelin basic protein (MBP) mutant that exhibits severe central nervous system (CNS) dysmyelination. We used a combination of immunohistochemistry, immunoblot and Northern blot analyses to determine the effect of MBP deficits on the expression of other CNS myelin genes in this mutant. Immunohistochemistry revealed a marked reduction in all major myelin proteins and differences in their intracellular distribution. Immunoblots confirmed the decreased expression of these proteins and indicated that relative levels of proteolipid protein (PLP) and DM20 were altered in this mutant. Quantitation of mRNA levels indicated that decreases in PLP and DM20 were a result of changes in mRNA levels but detected no change in other myelin gene transcripts.
长-伊文斯颤抖（les）大鼠是一种髓鞘碱性蛋白（MBP）突变体，表现出严重的中枢神经系统（CNS）脱髓鞘。我们结合使用免疫组织化学、免疫印迹和Northern印迹分析来确定MBP缺陷对该突变体中其他CNS髓鞘基因表达的影响。免疫组织化学显示所有主要髓鞘蛋白显著减少，且它们的细胞内分布存在差异。免疫印迹证实了这些蛋白表达的降低，并表明该突变体中蛋白脂蛋白（PLP）和DM20的相对水平发生了改变。mRNA水平定量分析表明，PLP和DM20的减少是mRNA水平变化的结果，但未检测到其他髓鞘基因转录本的变化。
