Tessele F, Englert G, Monteggia L O
Instituto de Pesquisas Hidráulicas, Universidade Federal do Rio Grande do Sul, Porto. Alegre, Brasil.
Water Sci Technol. 2002;46(1-2):253-6.
Biofilm development onto polypropylene particles (<4 mm) was studied in a laboratory-scale down flow anaerobic fluidised bed reactor. The reactor was fed with a synthetic solution containing sucrose and nutrients, and operated at 35 degrees C during 65 days at 44% bed expansion rate and 36 h HRT. Scanning electron microscopy (SEM) monitored the biofilm development. Initial adhesion occurred within the first 6 hours and after day 44 biofilm structure was complete. The presence of attached cells morphologically similar to Methanotrix bacilli and Methanosarcina sp. was observed by Scanning Electron Microscopy (SEM). The biofilm and the carrier surface roughness were measured by atomic force microscopy (AFM) and yielded 9.1 and 75 nm respectively. Results also showed good correlation between the SEM characterisation and the conventional anaerobic reactor parameters.
在实验室规模的下行流厌氧流化床反应器中，研究了生物膜在聚丙烯颗粒（<4毫米）上的形成情况。向反应器中加入含有蔗糖和营养物质的合成溶液，并在35摄氏度下运行65天，床层膨胀率为44%，水力停留时间为36小时。通过扫描电子显微镜（SEM）监测生物膜的形成。最初的粘附在最初6小时内发生，在第44天后生物膜结构完整。通过扫描电子显微镜（SEM）观察到存在形态上类似于甲烷杆菌属和甲烷八叠球菌属的附着细胞。通过原子力显微镜（AFM）测量生物膜和载体表面粗糙度，分别得到9.1纳米和75纳米。结果还表明，SEM表征与传统厌氧反应器参数之间具有良好的相关性。
