García-Morales J L, Romero L I, Sales D
Department of Chemical Engineering, Food Technology and Environmental Technologies, Faculty of Marine and Environmental Sciences, CASEM, University of Cádiz, Campus Rio San Pedro s/n. E-11510 Puerto Real Cádiz, Spain.
Water Sci Technol. 2003;47(5):197-200.
A key parameter in water and wastewater treatment technology is the biomass activity in terms of substrate removal ability. The effects of organic load rate and percentage of bed expansion on biofilm specific methanogenic activity were determined in an anaerobic fluidized bed reactor treating wine-distillery wastes in the thermophilic range (55 degrees C). The proposed activity tests are highly reproducible: an experiment with three identical tests has shown that the standard deviation with respect to the mean values is less than 3%. Specific tests are applied to measure the maximum methanogenic activities of the biomass carrier in lab-scale anaerobic biofilm reactors. These tests have been successfully applied for monitoring the support colonization process and the evolution of biofilm activity in reactors, anaerobic filter and fluidized bed, with different operating conditions. The results show a dependence between the percentage of bed expansion and the specific activity of methanogenic microbiote on biofilm. There is a relationship between the percentage of bed expansion, the sheer stress on the biofilm and the hydrodynamic conditions in the system. Initial biofilm detachment can be compensated with the increase of biomass and of its activity due to the reduction of the substrate diffusional limitations to the microorganism growth inside the support pores.
水和废水处理技术中的一个关键参数是生物量去除底物的活性。在处理葡萄酒厂废水的嗜热范围（55℃）厌氧流化床反应器中，测定了有机负荷率和床层膨胀百分比对生物膜特定产甲烷活性的影响。所提出的活性测试具有高度可重复性：一项包含三个相同测试的实验表明，相对于平均值的标准偏差小于3%。特定测试用于测量实验室规模厌氧生物膜反应器中生物质载体的最大产甲烷活性。这些测试已成功应用于监测不同运行条件下反应器、厌氧滤池和流化床中载体的定殖过程以及生物膜活性的演变。结果表明床层膨胀百分比与生物膜上产甲烷微生物群的比活性之间存在相关性。床层膨胀百分比、生物膜上的剪切应力与系统中的流体动力学条件之间存在关联。由于底物向载体孔隙内微生物生长的扩散限制降低，初始生物膜脱落可通过生物量及其活性的增加得到补偿。
