Rowan A K, Snape J R, Fearnside D, Curtis T P, Barer M R, Head I M
Centre of Molecular Ecology, University of Newcastle, Newcastle-upon-Tyne, UK.
Water Sci Technol. 2002;46(1-2):319-22.
Lab-scale reactors are commonly used to simulate full-scale plants as they permit the effects of defined experimental perturbations to be evaluated. Ideally, lab- and full-scale reactors should possess similar microbial populations. To determine this we compared the diversity of the beta-proteobacterial autotrophic ammonia-oxidising bacteria (AOB) in a full-scale and lab-scale biological aerated filter (BAF) using PCR with AOB selective primers combined with denaturing gradient gel electrophoresis (DGGE). PCR amplified 16S rRNA gene fragments from the nitrification unit of the lab-and full-scale BAF were subjected to cloning and sequencing to determine the phylogenetic affiliation of the AOB. A high degree of comparability between the lab-and full-scale BAF was observed with respect to AOB populations. However minor differences were apparent. The importance of these minor constituents in the overall performance of the reactor is unknown. Nonetheless the lab-scale reactor in this study did appear to reflect the dominant AOB community within the full-scale equivalent.
实验室规模的反应器通常用于模拟全尺寸工厂，因为它们能够评估特定实验扰动的影响。理想情况下，实验室规模和全尺寸反应器应具有相似的微生物群落。为了确定这一点，我们使用针对氨氧化细菌（AOB）的选择性引物结合变性梯度凝胶电泳（DGGE）的PCR技术，比较了全尺寸和实验室规模的生物曝气滤池（BAF）中β-变形菌自养氨氧化细菌（AOB）的多样性。从实验室规模和全尺寸BAF的硝化单元中PCR扩增的16S rRNA基因片段进行克隆和测序，以确定AOB的系统发育归属。在AOB种群方面，观察到实验室规模和全尺寸BAF之间具有高度的可比性。然而，也存在一些细微的差异。这些次要成分在反应器整体性能中的重要性尚不清楚。尽管如此，本研究中的实验室规模反应器似乎确实反映了全尺寸等效物中占主导地位的AOB群落。
