Oerther D B, van Loosdrecht M C M, Raskin L
Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana 61801, USA.
Water Sci Technol. 2002;46(1-2):443-7.
Batch growth studies with pure cultures of Acinetobacter johnsoniiT and Acinetobacter johnsonii strain 210 on various media formulations were used to examine the effects of micronutrient composition on the growth rate of microbial populations in wastewater treatment systems. On nutrient rich Luria-Bertani medium, both strains of A. johnsonii grew with a doubling time of approximately 30 min. On a defined, minimal medium with acetate as the sole carbon source, the doubling time of A. johnsoniiT was 9.62 h and the doubling time of strain 210a was 2.6 h. Using a synthetic wastewater as a growth medium, the type strain had a doubling time of 56 h and strain 210a had a doubling time of 9.62 h. The concentration and redox state of iron appeared to be the principle growth limiting factors with higher doubling times occurring in media containing ferric iron as compared to ferrous iron. Additionally, grab samples from batch growth experiments were analyzed with oligonucleotide hybridization probes targeting the mature 16S ribosomal RNA (rRNA) and precursor 16S rRNA of Acinetobacter spp. Results showed that the precursor 16S rRNA levels responded more rapidly and to a greater extent than total 16S rRNA levels to changes in the micronutrient composition of the growth media. Precursor 16S rRNA levels increased in both strains when overnight cultures were diluted with fresh media and when micronutrient supplements were added to growing cultures. Our results show that the micronutrient composition of the influent wastewater can have a significant impact on the microbial community structure in wastewater treatment systems.
利用约翰逊不动杆菌T和约翰逊不动杆菌210菌株的纯培养物在各种培养基配方上进行分批生长研究，以检验微量营养成分对废水处理系统中微生物种群生长速率的影响。在营养丰富的Luria-Bertani培养基上，两种约翰逊不动杆菌菌株的生长倍增时间约为30分钟。在以乙酸盐为唯一碳源的限定基本培养基上，约翰逊不动杆菌T的倍增时间为9.62小时，210a菌株的倍增时间为2.6小时。以合成废水作为生长培养基时，模式菌株的倍增时间为56小时，210a菌株的倍增时间为9.62小时。铁的浓度和氧化还原状态似乎是主要的生长限制因素，与含有亚铁的培养基相比，含有高铁的培养基中倍增时间更长。此外，使用针对不动杆菌属成熟16S核糖体RNA（rRNA）和前体16S rRNA的寡核苷酸杂交探针分析分批生长实验的抓取样本。结果表明，前体16S rRNA水平对生长培养基微量营养成分变化的反应比总16S rRNA水平更快、程度更大。当过夜培养物用新鲜培养基稀释以及向生长培养物中添加微量营养补充剂时，两种菌株的前体16S rRNA水平均升高。我们的结果表明，进水废水的微量营养成分可对废水处理系统中的微生物群落结构产生重大影响。
