膳食反式- vaccenic酸（反式11-18:1）可提高泌乳小鼠和哺乳幼崽组织中顺式9，反式11-共轭亚油酸（瘤胃酸）的浓度。

Dietary trans-vaccenic acid (trans11-18:1) increases concentration of cis9,transll-conjugated linoleic acid (rumenic acid) in tissues of lactating mice and suckling pups.

作者信息

Loor Juan J, Lin Xiaobo, Herbein Joseph H

机构信息

Dairy Science Department, Virginia Tech, Blacksburg 24061-0315, USA.

出版信息

Reprod Nutr Dev. 2002 Mar-Apr;42(2):85-99. doi: 10.1051/rnd:2002009.

DOI:10.1051/rnd:2002009

PMID:12216963

Abstract

Lactating mice were fed trans-vaccenic acid (trans 11-18:1, TVA) to assess desaturation of TVA to cis9,trans11-conjugated linoleic acid (9/11CLA). Diets contained 30 g x kg(-1) 18:2n-6 (LA) or 20 g LA plus 10 g 18:0 (SA), TVA, or a CLA mixture (MCLA). Compared with SA, feeding TVA increased 9/11CLA concentrations in blood plasma phospholipid, triglyceride, and free fatty acid fractions. However, concentrations of 9/11CLA in plasma fractions were greater when MCLA was fed compared with SA or TVA. No 9/11CLA was detected in liver of mice fed SA, and it was only 1 mg x g(-1) of total fatty acids in the carcass. In contrast, 9/11CLA content of liver (5 mg x g(-1)) and carcass (6 mg x g(-1)) of mice fed TVA was similar to liver (5 mg x g(-1)) and carcass (7 mg x g(-1)) of mice fed MCLA. Mammary tissue of SA-fed mice had no detectable 9/11 CLA, compared with 5 or 14 mg x g(-1) for TVA or MCLA-fed mice. Stearoyl-CoA desaturase activity in mammary tissue from TVA-fed dams was 14% greater compared with SA. Activity of this enzyme in liver tissue was similar among treatments. In pups nursing TVA-fed dams, 9/1 ICLA accounted for 3 mg x g(-1) in liver but no 9/11CLA was detected in the carcass. In pups nursing MCLA-fed dams, however, 9/11CLA accounted for 8 and 6 mg x g(-1) in liver and carcass. Results indicated TVA desaturation enhanced 9/11CLA in tissues and milk fat.

摘要

给哺乳期小鼠喂食反式vaccenic酸（反式11-18:1，TVA），以评估TVA去饱和生成顺-9，反-11-共轭亚油酸（9/11CLA）的情况。日粮含有30 g·kg⁻¹的18:2n-6（LA）或20 g LA加10 g 18:0（SA）、TVA或CLA混合物（MCLA）。与SA相比，喂食TVA可提高血浆磷脂、甘油三酯和游离脂肪酸组分中9/11CLA的浓度。然而，与SA或TVA相比，喂食MCLA时血浆组分中9/11CLA的浓度更高。喂食SA的小鼠肝脏中未检测到9/11CLA，其在胴体总脂肪酸中仅为1 mg·g⁻¹。相比之下，喂食TVA的小鼠肝脏（5 mg·g⁻¹）和胴体（6 mg·g⁻¹）中9/11CLA的含量与喂食MCLA的小鼠肝脏（5 mg·g⁻¹）和胴体（7 mg·g⁻¹）相似。喂食SA的小鼠乳腺组织中未检测到9/11CLA，而喂食TVA或MCLA的小鼠为5或14 mg·g⁻¹。与SA相比，喂食TVA的母鼠乳腺组织中硬脂酰辅酶A去饱和酶活性高14%。各处理组肝脏组织中该酶的活性相似。在吸食喂食TVA母鼠乳汁的幼崽中，9/11CLA在肝脏中占3 mg·g⁻¹，但在胴体中未检测到。然而，在吸食喂食MCLA母鼠乳汁的幼崽中，9/11CLA在肝脏和胴体中分别占8和6 mg·g⁻¹。结果表明，TVA去饱和增强了组织和乳脂肪中的9/11CLA。