用于生产重组变应原的表达系统。

Expression systems for production of recombinant allergens.

作者信息

Schmidt M, Hoffman D R

机构信息

Department of Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USA.

出版信息

Int Arch Allergy Immunol. 2002 Aug;128(4):264-70. doi: 10.1159/000063865.

DOI:10.1159/000063865

PMID:12218364

Abstract

Recombinant allergenic proteins have been produced in a variety of different expression systems. This review gives examples of and compares prokaryotic expression systems, such as Escherichia coli, and eukaryotic systems including the yeasts, Saccharomyces cerevisiae and Pichia pastoris, baculovirus-insect cell systems, mammalian cell systems and plant systems. Important factors to consider in choosing an expression system are discussed and examples of applications are given.

摘要

重组变应原蛋白已在多种不同的表达系统中产生。本综述给出了原核表达系统（如大肠杆菌）以及真核系统（包括酵母、酿酒酵母和毕赤酵母、杆状病毒-昆虫细胞系统、哺乳动物细胞系统和植物系统）的示例并进行了比较。讨论了选择表达系统时需要考虑的重要因素，并给出了应用示例。