经鼻用抗原和CpG寡脱氧核苷酸免疫后大鼠泪液IgA抗体反应增强。

Enhancement of rat tear IgA antibody responses following intranasal immunization with antigen and CpG ODN.

作者信息

Gill Randall F, Montgomery Paul C

机构信息

Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI 48201, USA.

出版信息

Curr Eye Res. 2002 Mar;24(3):228-33. doi: 10.1076/ceyr.24.3.228.8298.

DOI:10.1076/ceyr.24.3.228.8298

PMID:12221533

Abstract

PURPOSE

To determine the effect of unmethylated oligodeoxynucleotides containing bacterial CpG motifs (CpG ODN) on the induction of rat tear IgA antibody responses.

METHODS

Rats were immunized intranasally with either soluble dinitrophenylated bovine serum albumin (DNP-BSA) or poly(lactide-co-glycolide) (PLG) encapsulated DNP-BSA in combination with CpG ODN. The animals received two immunizations 21 days apart. Following the second immunization, tear, saliva and serum samples were collected for 28 days and analyzed for antigen specific antibodies. Tear IgA, saliva IgA and serum IgG antibody concentrations were determined by ELISA.

RESULTS

Co-administration of CpG ODN with either soluble or encapsulated antigen resulted in significantly elevated levels of both tear and salivary IgA antibodies as well as levels of serum IgG antibodies. Microencapsulated DNP-BSA plus CpG ODN elicited higher levels of IgA antibodies in tears than did soluble antigen plus CpG ODN.

CONCLUSIONS

CpG ODN is an effective mucosal immune modulator for enhancing rat tear IgA antibody responses to both soluble and microencapsulated antigens.

摘要

目的

确定含有细菌CpG基序的未甲基化寡脱氧核苷酸（CpG ODN）对大鼠泪液IgA抗体反应诱导的影响。

方法

用可溶性二硝基苯基化牛血清白蛋白（DNP-BSA）或聚（丙交酯-共-乙交酯）（PLG）包裹的DNP-BSA联合CpG ODN经鼻免疫大鼠。动物在21天的间隔内接受两次免疫。第二次免疫后，收集泪液、唾液和血清样本28天，并分析抗原特异性抗体。通过ELISA测定泪液IgA、唾液IgA和血清IgG抗体浓度。