皮质醇对源自兔直肠肺和皮肤的连续传代成纤维细胞培养物的影响。

Effects of cortisol on serially propogated fibroblast cell cultures derived from the rabbit retal lung and skin.

作者信息

Smith B T, Giroud C J

出版信息

Can J Physiol Pharmacol. 1975 Dec;53(6):1037-41. doi: 10.1139/y75-144.

Abstract

Cortisol affects the growth of serially propogated, fibroblast cell cultures derived from the rabbit fetal lung in a manner which is dependent upon the gestational age of the material used: early in gestation (20 days), the hormone (10(-7)-10(-5) M) stimulates [6-3H]thymidine incorporation into DNA, while in late gestation (28 days), cortisol (10(-7) and 10(-6) M) inhibits this process. Cultures derived from the rabbit fetal skin are inhibited by cortisol (10(-5) M) at both gestational ages. Fibroblasts derived from lung, but not from skin, efficiently convert cortisone to cortisol and this activity increases with advancing gestation. Cortisol does not affect the incorporation of [3H]choline into lecithin by confluent cultures of any of the fibroblast types studied.

摘要

皮质醇对源自兔胎儿肺的连续传代的成纤维细胞培养物的生长有影响，其方式取决于所用材料的胎龄：在妊娠早期（20天），该激素（10⁻⁷ - 10⁻⁵ M）刺激[6 - ³H]胸腺嘧啶核苷掺入DNA，而在妊娠后期（28天），皮质醇（10⁻⁷和10⁻⁶ M）抑制这一过程。在两个胎龄时，源自兔胎儿皮肤的培养物均受到皮质醇（10⁻⁵ M）的抑制。源自肺而非皮肤的成纤维细胞能有效地将可的松转化为皮质醇，且这种活性随胎龄增加而增强。皮质醇不影响所研究的任何一种成纤维细胞类型的汇合培养物将[³H]胆碱掺入卵磷脂的过程。