Shimada Yasuhiko, Morita Tatsuya, Sugiyama Kimio
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Suzuoka, Japan.
Biosci Biotechnol Biochem. 2002 Jul;66(7):1605-9. doi: 10.1271/bbb.66.1605.
Effects of dietary eritadenine on liver microsomal delta6-desaturase activity and the fatty acid profile of phosphatidylcholine, cholesteryl esters, and triglycerides of liver microsomes or plasma were investigated in rats fed different fats (palm oil, olive oil, and safflower oil). The activity of delta6-desaturase was influenced by both dietary fat types and eritadenine. In rats fed control diets, delta6-desaturase activity was higher in the order of the palm oil, olive oil, and safflower oil groups. In rats fed eritadenine-supplemented diets, the enzyme activity was markedly decreased to a constant level irrespective of dietary fat type. The 20:4n-6/18:2n-6 ratio of phosphatidylcholine and cholesteryl esters, as compared with triglycerides, was highly sensitive to eritadenine. The results suggest that the activity of delta6-desaturase is regulated by dietary fats and eritadenine independently, and that the effect of eritadenine is stronger than that of dietary fats.
在喂食不同脂肪（棕榈油、橄榄油和红花油）的大鼠中，研究了膳食中的香菇嘌呤对肝脏微粒体δ6-去饱和酶活性以及肝脏微粒体或血浆中磷脂酰胆碱、胆固醇酯和甘油三酯的脂肪酸谱的影响。δ6-去饱和酶的活性受膳食脂肪类型和香菇嘌呤的影响。在喂食对照饮食的大鼠中，δ6-去饱和酶活性按棕榈油组、橄榄油组和红花油组的顺序升高。在喂食补充香菇嘌呤饮食的大鼠中，无论膳食脂肪类型如何，酶活性均显著降低至恒定水平。与甘油三酯相比，磷脂酰胆碱和胆固醇酯的20:4n-6/18:2n-6比值对香菇嘌呤高度敏感。结果表明，δ6-去饱和酶的活性分别受膳食脂肪和香菇嘌呤的调节，且香菇嘌呤的作用强于膳食脂肪。
