Tkachenko M M, Sahach V F, Kotsiuruba A V, Baziliuk O V, Bukhanevych O M, Mehed' O F, Stepanenko L H
A.A. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kiev.
Fiziol Zh (1994). 2002;48(4):3-13.
Endothelium-dependent and endothelium-independent reactions of vascular smooth muscles were studied in isolated preparations of thoracic part of aorta and portal vein of rats of two age groups--6.8 mo. (control) and 24-26 mo. (old animals). In old rats the disturbances occurred primarily in endothelium-dependent vascular reactions. This age-related dysfunction of endothelium resulted in inhibition of NO-dependent regulation of vascular tone. Age-related changes in the state of plasmic membranes of erythrocytes were established using a method of acid hemolysis. Age-related changes in the levels of active forms of oxygen (.O2-, H2O2, .OH-) and nitrogen (NO2-, NO3-) were determined in the myocardium, aorta, blood plasma and erythrocytes of adult and old animals.
在两个年龄组大鼠（6-8个月龄，为对照组；24-26个月龄，为老年动物）的离体胸主动脉和门静脉标本中，研究了血管平滑肌的内皮依赖性和非内皮依赖性反应。老年大鼠主要在内皮依赖性血管反应中出现紊乱。这种与年龄相关的内皮功能障碍导致血管张力的一氧化氮依赖性调节受到抑制。采用酸溶血法确定了红细胞质膜状态的年龄相关变化。测定了成年和老年动物心肌、主动脉、血浆和红细胞中活性氧（超氧阴离子、过氧化氢、羟自由基）和活性氮（亚硝酸根、硝酸根）水平的年龄相关变化。
