Study of the coordination of amino acids with metals using [trans-en2Os(eta2-H2)L]2+ as a 1H NMR probe.

This is a study of the interaction of 23 kinds of amino acids, peptides and their analogues with Os((II)) at different pD values. Experiments show that in acidic conditions, the carboxyl group in amino acids can coordinate with Os((II)), and there exists H-D coupling of the dihydrogen of the probe with D2O in strongly acidic conditions, N does not coordinate with Os((II)); In alkaline conditions, the carboxyl group can coordinate with Os, and the coordinating species have trans and cis isomers, and the trans isomer can convert to cis with time; N of -NH2- in alpha-amino group can coordinate with Os((II)) while that in gamma-amino-n-butyric acid cannot do that. Since the target of some anti tumor agents are nucleic acids and proteins, we demonstrate a competitive mode to study how the anti tumor complex Me2SnCl2 binds to amino acid Ala, and the minimum binding amount and formation constant of the metal anti tumor metal complexes binding with amino acid are also obtained.