Kuzma J., Fall R.
Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215.
Plant Physiol. 1993 Feb;101(2):435-440. doi: 10.1104/pp.101.2.435.
Isoprene (2-methyl-1,3-butadiene) is a major volatile hydrocarbon produced by many plant species. Here we report that in velvet bean (Mucuna sp.), isoprene emission is strongly dependent on leaf developmental state and that changes in extractable isoprene synthase activity parallel isoprene emission rates during leaf development. Both leaf emission and enzyme activity exhibit over 100-fold increases from leaf emergence to leaf age 14 d and exhibit similar patterns to 23 d. This suggests that the enzyme, isoprene synthase, is responsible for the in vivo production of isoprene and that the level of the enzyme regulates the pattern of isoprene emission in response to leaf development.
异戊二烯(2-甲基-1,3-丁二烯)是许多植物物种产生的一种主要挥发性碳氢化合物。在此我们报告,在藜豆(黎豆属)中,异戊二烯排放强烈依赖于叶片发育状态,并且在叶片发育过程中,可提取的异戊二烯合酶活性的变化与异戊二烯排放速率平行。从叶片出现到叶龄14天,叶片排放和酶活性均增加了100多倍,并且在23天时呈现出相似的模式。这表明异戊二烯合酶这种酶负责异戊二烯的体内产生,并且该酶的水平响应叶片发育调节异戊二烯排放模式。