Eskew D. L., Jiang Q., Caetano-Anolles G., Gresshoff P. M.
Plant Molecular Genetics, Institute of Agriculture and Center for Legume Research, University of Tennessee, P.O. Box 1071, Knoxville, Tennessee 37901-1071.
Plant Physiol. 1993 Dec;103(4):1139-1145. doi: 10.1104/pp.103.4.1139.
Nodule development in the interaction of Glycine soja Sieb. & Zucc. PI468.397 with Bradyrhizobium japonicum USDA110 was studied by hypochlorite clearing and methylene blue staining. Even the earliest stages of nodule development could be observed. The entire length of the primary root was examined up to 15 d postinoculation. Markedly curled root hairs and the first cell divisions in the hypodermal layer (stage I) were observed 2 d postinoculation, and by 3 d cell division activity had spread to the outer layers of the cortex (stage II). Cortical cell division centers not associated with curled root hairs, frequently observed in soybean (Glycine max [L.] Merr.), were very rare in G. soja. The cortical cell division centers that had developed a well-defined nodule meristem (at or beyond stage IV) by 6 d postinoculation continued to develop, but the less-advanced stages became arrested. Almost all nodules developed near the position of the root tip at the time of inoculation. In the parts of the root that developed after inoculation, regions with a high density of markedly curled root hairs per root length were observed. The percentage of the curled root hairs associated with cortical cell division centers, however, declined with each successive peak. Regulation of nodule development in G. soja was similar to that previously reported in soybean, although the rate of nodule development was slower.
通过次氯酸盐透明法和亚甲基蓝染色法研究了野生大豆（Glycine soja Sieb. & Zucc. PI468.397）与慢生根瘤菌（Bradyrhizobium japonicum USDA110）相互作用过程中的根瘤发育情况。甚至可以观察到根瘤发育的最早阶段。对接种后15天内的主根全长进行了检查。接种后2天观察到明显卷曲的根毛以及皮层下细胞的首次分裂（I期），到3天时细胞分裂活动已扩展到皮层外层（II期）。在大豆（Glycine max [L.] Merr.）中经常观察到的与卷曲根毛无关的皮层细胞分裂中心，在野生大豆中非常罕见。接种后6天已形成明确根瘤分生组织（IV期或更晚）的皮层细胞分裂中心继续发育，但较早期阶段则停滞。几乎所有根瘤都在接种时根尖的位置附近形成。在接种后发育的根段中，观察到每根长度上明显卷曲根毛密度较高的区域。然而，与皮层细胞分裂中心相关的卷曲根毛百分比随着每个连续峰值而下降。野生大豆根瘤发育的调控与先前在大豆中报道的相似，尽管根瘤发育速率较慢。
