Rana Debashis, Krishnan Hari B
Department of Plant Microbiology and Pathology, University of Missouri, Columbia, MO 65211, USA.
Curr Microbiol. 2002 Nov;45(5):378-82. doi: 10.1007/s00284-002-3772-z.
Rhizobium sp. SIN-1, a nitrogen-fixing symbiont of Sesbania aculeata and other tropical legumes, carries two copies of nodD, both on a sym plasmid. We have isolated these two nodD genes by screening a genomic library of Rhizobium sp. SIN-1 with a nodD probe from Sinorhizobium meliloti. Nucleotide sequence and the deduced amino acid sequence analysis indicated that the nodD genes of Rhizobium sp. SIN-1 are most closely related to those of R. tropici and Azorhziobium caulinodans. Rhizobium sp. SIN-1 nodD1 complemented a S. meliloti nodD1 D2 D3 negative mutant for nodulation on alfalfa, but failed to complement a nodD1 mutant of S. fredii USDA191 for soybean nodulation. A hybrid nodD gene, containing the N-terminus of S. fredii USDA191 nodD1 and the C-terminus of Rhizobium sp. SIN-1 nodD1, complemented the nodD1 negative mutant of USDA191 for nodulation on soybean.
刺槐根瘤菌SIN-1是多刺田菁和其他热带豆科植物的固氮共生体，在共生质粒上携带两个拷贝的nodD基因。我们通过用来自苜蓿中华根瘤菌的nodD探针筛选刺槐根瘤菌SIN-1的基因组文库，分离出了这两个nodD基因。核苷酸序列和推导的氨基酸序列分析表明，刺槐根瘤菌SIN-1的nodD基因与热带根瘤菌和茎瘤固氮根瘤菌的nodD基因关系最为密切。刺槐根瘤菌SIN-1 nodD1可互补苜蓿中华根瘤菌nodD1 D2 D3阴性突变体在苜蓿上结瘤，但不能互补费氏中华根瘤菌USDA191的nodD1突变体在大豆上结瘤。一个包含费氏中华根瘤菌USDA191 nodD1的N端和刺槐根瘤菌SIN-1 nodD1的C端的杂交nodD基因，可互补USDA191的nodD1阴性突变体在大豆上结瘤。
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