[费氏中华根瘤菌042BS调控基因的克隆与功能分析]
[The cloning and functional analysis of Sinorhizobium fredii 042BS regulatory modulation genes].
作者信息
Zhang Haiyu, Zhang Haiyu, Li Xiaohong, Wen Shangkun, Yan Susheng
机构信息
Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
出版信息
Wei Sheng Wu Xue Bao. 2002 Feb;42(1):33-9.
The total DNA of Sinorhizobium fredii 042BS was digested by EcoRI for Southern blotting with probes of nodD1 and nodD2 from S. fredii USDA257. The 3 kb positive band hybrided with nodD1 probe and 6 kb positive band with nodD2 probe were found, respectively. Partial gene library were constructed using pUC18 as vector, and the clones with the nodD1 and nodD2 genes were obtained. The sequence of nodD1 and nodD2 of 042B showed that they are highly homologous with nodD1 and nodD2 of S. fredii. The fragment with nodD1 was cloned into the vector pBBRIMCS-5 and introduced into R. leguminosarum bv. viciae LPR5054 to study the function of the nodD1. The results showed that nodD1 of 042B can be induced by genistein and luteolin secreted by the seedlings of soybean and alfalfa respectively.
用EcoRI消化费氏中华根瘤菌042BS的总DNA，以费氏中华根瘤菌USDA257的nodD1和nodD2为探针进行Southern杂交。分别发现与nodD1探针杂交的3 kb阳性条带和与nodD2探针杂交的6 kb阳性条带。以pUC18为载体构建部分基因文库，获得了含有nodD1和nodD2基因的克隆。042B的nodD1和nodD2序列表明它们与费氏中华根瘤菌的nodD1和nodD2高度同源。将含有nodD1的片段克隆到载体pBBRIMCS-5中，并导入豌豆根瘤菌蚕豆生物型LPR5054中研究nodD1的功能。结果表明，042B的nodD1分别可被大豆和苜蓿幼苗分泌的染料木黄酮和木犀草素诱导。
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