Ogawa H, Goldsmith L A
J Invest Dermatol. 1977 Jan;68(1):32-5. doi: 10.1111/1523-1747.ep12485152.
A monospecific antibody for human epidermal transglutaminase was prepared in rabbits. The antibody formed single immunoprecipitin lines with purified or crude human transglutaminases and quantitatively precipitated transglutaminase activity. There were no precipitin reactions between human factor XIII (zymogen or active enzyme) and antihuman epidermal transglutaminase or between human epidermal transglutaminase and antihuman factor XIII. Heating epidermal transglutaminase (56 degrees C, 15 min) in the presence of calcium increased the enzyme activity up to 10 times baseline levels. The heat-activated human epidermal transglutaminase was identical by immunodiffusion with the native enzyme, although slightly higher precipitation titers were detected following heating. There was no cross-reaction of antihuman epidermal transglutaminase with frog, rat, mouse, chicken, or human hair follicle transglutaminases.
用兔制备了一种针对人表皮转谷氨酰胺酶的单特异性抗体。该抗体与纯化的或粗制的人转谷氨酰胺酶形成单一免疫沉淀线，并能定量沉淀转谷氨酰胺酶活性。人因子XIII（酶原或活性酶）与人表皮转谷氨酰胺酶抗体之间或人表皮转谷氨酰胺酶与人因子XIII抗体之间均无沉淀反应。在有钙存在的情况下加热表皮转谷氨酰胺酶（56℃，15分钟）可使酶活性提高至基线水平的10倍。经热激活的人表皮转谷氨酰胺酶通过免疫扩散法检测与天然酶相同，尽管加热后检测到的沉淀效价略高。抗人表皮转谷氨酰胺酶与蛙、大鼠、小鼠、鸡或人毛囊转谷氨酰胺酶无交叉反应。
