使用终止子引物和模块化载体进行快速基因克隆。
Rapid gene cloning using terminator primers and modular vectors.
作者信息
Donahue William F, Turczyk Brian M, Jarrell Kevin A
机构信息
Department of Pharmacology and Experimental Therapeutics, Boston University Medical Center, 80 East Concord Street, Boston, MA 02118, USA.
出版信息
Nucleic Acids Res. 2002 Sep 15;30(18):e95. doi: 10.1093/nar/gnf094.
Abstract
We seek to create useful biological diversity by exploiting the modular nature of genetic information. In this report we describe experiments that focus on the modular nature of plasmid cloning vectors. Bacterial plasmids are modular entities composed of origins of replication, selectable markers and other components. We describe a new ligation-independent cloning method that allows for rapid and seamless assembly of vectors from component modules. We further demonstrate that gene cloning can be accomplished simultaneously with assembly of a modular vector. This approach provides considerable flexibility as it allows for 'menu driven' cloning of genes into custom assembled modular vectors.
摘要
我们试图通过利用遗传信息的模块化特性来创造有用的生物多样性。在本报告中，我们描述了聚焦于质粒克隆载体模块化特性的实验。细菌质粒是由复制起点、选择标记和其他组件组成的模块化实体。我们描述了一种新的不依赖连接的克隆方法，该方法能够从组件模块快速无缝地组装载体。我们进一步证明，基因克隆可以与模块化载体的组装同时完成。这种方法提供了相当大的灵活性，因为它允许将基因 “菜单驱动” 式地克隆到定制组装的模块化载体中。
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