Xu Wei-Hua, Yamashita Okitsugu
The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212000, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(2):214-217.
Embryonic diapause and sex pheromone biosynthesis in the silkworm, Bombyx mori, are induced by the diapause hormone (DH) and the pheromone biosynthesis activating neuropeptide (PBAN), respectively. These are produced in the suboesophageal ganglion from a common polyprotein precursor (DH-PBAN precursor) encoded in a single gene (DH-PBAN gene). Using RT-PCR and Southern hybridization methods, we quantitatively measured DH-PBAN mRNA content throughout the post-embryonic development. The results show that DH-PBAN gene not only expresses during pupal stage, but also during larval and adult stages. The high level of expression during pupal stage suggests that DH and PBAN are respectively synthesized.
家蚕的胚胎滞育和性信息素生物合成分别由滞育激素（DH）和信息素生物合成激活神经肽（PBAN）诱导。它们由位于咽下神经节的一个单一基因（DH-PBAN基因）编码的共同多蛋白前体（DH-PBAN前体）产生。我们使用逆转录聚合酶链反应（RT-PCR）和Southern杂交方法，定量测定了胚胎后期发育过程中DH-PBAN mRNA的含量。结果表明，DH-PBAN基因不仅在蛹期表达，在幼虫期和成虫期也有表达。蛹期的高表达水平表明DH和PBAN是分别合成的。
