Jahr Jonathan S, Lurie Fedor, Gosselin Robert, Lin Jonathan S, Wong Luchin, Owings John T, Larkin Edward
Department of Anesthesiology, University of California Los Angeles, Los Angeles, California 90095-1770, USA.
Am J Ther. 2002 Sep-Oct;9(5):431-6. doi: 10.1097/00045391-200209000-00011.
Polymerized bovine hemoglobin is currently under investigation as an alternative to blood-banked human red blood cells. Because of the dark red hemolyzed appearance of hemoglobin-based oxygen carrier (HBOC)-201, we sought to describe the effects of HBOC-201 on coagulation analyzers that perform prothrombin times, activated partial thromboplastin times, fibrinogen, and antithrombin. Pooled normal plasma was combined with HBOC-201 to achieve plasma hemoglobin levels of 1.4, 2.6, 3.8, 4.8, and 6.2 g/dL. Results for each test using HBOC-201-prepared plasma were compared with results using saline-matched controls. Two consecutive absolute result differences of >10% between saline controls and HBOC-201 samples were used for determining interference in test accuracy by the concentration of HBOC-201. Mechanical detection methods (fibrometer, STA, CS-190) and the MDA-180 method were less affected by increasing levels of HBOC-201 than optical detection devices for all test parameters.
聚合牛血红蛋白目前正在作为血库中人类红细胞的替代品进行研究。由于基于血红蛋白的氧载体（HBOC）-201呈现暗红色溶血外观，我们试图描述HBOC-201对进行凝血酶原时间、活化部分凝血活酶时间、纤维蛋白原和抗凝血酶检测的凝血分析仪的影响。将混合的正常血浆与HBOC-201混合，使血浆血红蛋白水平达到1.4、2.6、3.8、4.8和6.2 g/dL。将使用HBOC-201制备的血浆进行每项检测的结果与使用生理盐水匹配对照的结果进行比较。生理盐水对照与HBOC-201样本之间连续两次绝对结果差异>10%，用于确定HBOC-201浓度对检测准确性的干扰。对于所有检测参数，机械检测方法（纤维蛋白仪、STA、CS-190）和MDA-180方法受HBOC-201水平升高的影响小于光学检测设备。
Zotero 插件