Sato T, Fujii T, Kojima K
Physiol Chem Phys. 1975;7(6):523-8.
Shape change of human erythrocyte due to the membrane externalization induced by incorporation of excess lysolecithin or by hypertonic treatment in vitro, was accompanied by a decrease in the electrophoretic mobility of the cell. No change in sialic acid content of the membrane, mostly responsible for the cell surface charge, was observed in any of these treatments. Therefore, the membrane externalization seems to cause a redistribution of the surface charge and the resulting alteration of the electrophoretic mobility of the cell. Heating at 48 degrees C for 1 hour induced both the shape change and change in the mobility, but at the same time it reduced the membrane sialic acid content.
在体外，通过掺入过量溶血卵磷脂或进行高渗处理诱导膜外化，导致人红细胞形状改变，同时细胞的电泳迁移率降低。在任何一种处理中，均未观察到膜中唾液酸含量的变化，而唾液酸含量是细胞表面电荷的主要决定因素。因此，膜外化似乎导致表面电荷重新分布，进而改变细胞的电泳迁移率。在48摄氏度加热1小时会同时引发形状改变和迁移率变化，但与此同时会降低膜中唾液酸含量。
