Dube J Y, Chapdelaine P, Dionne F T, Cloutier D, Tremblay R R
J Clin Endocrinol Metab. 1978 Jul;47(1):41-5. doi: 10.1210/jcem-47-1-41.
We have found a specific binding protein for synthetic progestins 6,7-[3H]methyltrienolone (R1881) and 17,21-dimethyl-19-norpregna-4,9-diene-3,20-dione (R5020) and in the testis cytosol from three "sisters" with the complete form of the testicular feminization syndrome. The binding component sediments in the 8S region of sucrose gradients. It is saturable. The apparent affinity constant (Ka) for R5020 was determined in two cases and found to be 1.8 and 0.6 X 10(8) M-1. The number of binding sites calculated from Scatchard plots is relatively high: 572 and 826 fmol/mg protein. Competition studies indicate that this putative receptor is specific for natural and synthetic progestins but not for 5 alpha-dihydrotestosterone and cortisol. Similar progestin binding could not be found in normal human and rat testes.
我们在三名患有完全型睾丸女性化综合征的“姐妹”的睾丸胞质溶胶中，发现了一种与合成孕激素6,7-[³H]甲基三烯诺龙（R1881）和17,21-二甲基-19-去甲孕-4,9-二烯-3,20-二酮（R5020）特异性结合的蛋白。该结合成分在蔗糖梯度的8S区域沉降。它具有饱和性。在两个病例中测定了R5020的表观亲和常数（Ka），分别为1.8和0.6×10⁸ M⁻¹。根据Scatchard图计算出的结合位点数相对较高：572和826 fmol/mg蛋白。竞争研究表明，这种假定的受体对天然和合成孕激素具有特异性，但对5α-二氢睾酮和皮质醇没有特异性。在正常人和大鼠的睾丸中未发现类似的孕激素结合。
