Nevalainen T J
Res Exp Med (Berl). 1975 Jul 14;165(2):163-8. doi: 10.1007/BF01854842.
Inbred BALB/c mice were given vinblastine sulfate (VelbeR) and/or pilocarpine hydrochloride (30 mg/kg) intraperitoneally and amylase activity was determined in the pancreas homogenate. When vinblastine was given 5 hours before measuring the pancreatic amylase content it inhibited the pilocarpine stimulated (2 hours) pancreatic secretion in a dosis-dependent fashion at doses 4, 40 and 400 mg/kg. Vinblastine (200 mg/kg) also delayed amylase accumulation during 3 hours in mice given vinblastine 2 hours after pilocarpine stimulation. The results support the hypothesis that faultless function and integrity of microtubules might be a prerequisite of pancreatic acinar cell secretion in vivo. Other effects of vinblastine, such as interference with amino acid metabolism, anticholinergic action, and cell injury must also be considered when interpreting the present findings form the point of view of the secretory mechanism of the pancreatic acinar cells.
将硫酸长春碱（VelbeR）和/或盐酸毛果芸香碱（30毫克/千克）腹腔注射给予近交系BALB/c小鼠，并测定胰腺匀浆中的淀粉酶活性。当在测量胰腺淀粉酶含量前5小时给予长春碱时，它以剂量依赖性方式抑制毛果芸香碱刺激（2小时）的胰腺分泌，剂量分别为4、40和400毫克/千克。长春碱（200毫克/千克）在毛果芸香碱刺激后2小时给予小鼠时，也会在3小时内延迟淀粉酶的积累。这些结果支持这样的假设，即微管的正常功能和完整性可能是体内胰腺腺泡细胞分泌的先决条件。从胰腺腺泡细胞分泌机制的角度解释当前发现时，还必须考虑长春碱的其他作用，如干扰氨基酸代谢、抗胆碱能作用和细胞损伤。
