Bogdanov Iu F, Dadashev S Ia, Grishaeva T M
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia.
Genetika. 2002 Aug;38(8):1078-89.
The published principles of computer analysis of genomes and protein sets in taxonomically distant eukaryotes are expounded. The authors developed a search strategy to identify in genomes of such organisms genes and proteins nonhomologous in primary structure but having similar functions in cells dividing by meiosis. This strategy based on the combined principles of genomics, proteomics, and morphometric analysis of subcellular structures was applied to a computer search for genes encoding the proteins of synaptonemal complexes in genomes of Drosophila melanogaster, the nematode Caenorhabditis elegans, and the plant Arabidopsis thaliana. These proteins proved to be functionally similar to their counterparts in yeast Saccharomyces cerevisiae (protein Zip1p) and mammals (protein SCP1).
阐述了已发表的关于分类学上相距较远的真核生物基因组和蛋白质组计算机分析的原理。作者开发了一种搜索策略，以在这些生物的基因组中识别出在一级结构上非同源但在减数分裂细胞中具有相似功能的基因和蛋白质。这种基于基因组学、蛋白质组学和亚细胞结构形态计量分析综合原理的策略，被应用于在黑腹果蝇、秀丽隐杆线虫和拟南芥基因组中进行计算机搜索，以寻找编码联会复合体蛋白质的基因。结果证明，这些蛋白质在功能上与其在酿酒酵母（蛋白质Zip1p）和哺乳动物（蛋白质SCP1）中的对应物相似。
