Kretova O V, Sokolova M A, Churikov N A
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
Genetika. 2002 Aug;38(8):1090-6.
To analyze the copies of the suffix short retro-element, its homologs were sought in nucleic acid sequence databases of the Drosophila melanogaster genome. The search yielded several conserved (near identical in sequence) copies, which are indicative of recent suffix transposition, and numerous divergent copies, which suggest ancient suffix transposition. Analysis of the short suffix ORF revealed a conserved protein domain, which was also found as the eighth C-terminal domain in reverse transcriptases of certain long interspersed elements (LINEs). The suffix-encoded polypeptide proved to be homologous to DNA- and RNA-recognizing domains.
为了分析后缀短反转录元件的拷贝，在黑腹果蝇基因组的核酸序列数据库中寻找其同源物。搜索产生了几个保守的（序列近乎相同）拷贝，这表明最近有后缀转座，以及许多不同的拷贝，这表明是古老的后缀转座。对短后缀开放阅读框的分析揭示了一个保守的蛋白质结构域，在某些长散在元件（LINEs）的逆转录酶中也发现它作为第八个C末端结构域。后缀编码的多肽被证明与DNA和RNA识别结构域同源。
