来自球形黑粉菌的核糖核酸酶U2的二硫键。

The disulfide bridges of ribonuclease U2 from Ustilago sphaerogena.

作者信息

Sato S, Uchida T

出版信息

J Biochem. 1975 Jun;77(6):1171-6.

Abstract

RNase U2 was partially hydrolyzed with chymotrypsin [EC 3.4.21.1] and sulfuric acid, and in each case the resulting peptides were separated by gel filtration, ion exchange column chromatography and paper electrophoresis. From the results of amino acid analysis of cystine-containing peptides and their oxidized components, the three disulfide bridges were located between the cystine residues at positions 1 and 53, 9 and 112, and 54 and 95.

摘要

核糖核酸酶U2用胰凝乳蛋白酶[EC 3.4.21.1]和硫酸进行部分水解，在每种情况下，所得肽通过凝胶过滤、离子交换柱色谱和纸电泳进行分离。根据含胱氨酸肽及其氧化成分的氨基酸分析结果，三个二硫键位于第1和53位、第9和112位以及第54和95位的胱氨酸残基之间。