文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

J Biochem. 1975 Jun;77(6):1193-7.

Tubulin purified from porcine brain in the presence of GTP contained 0.16 mole of GDP and 0.73 mole of GTP per 60,000 g of protein. 2. Microtubules reconstituted from the purified tubulin contained 0.43 mole of GDP and 0.41 mole of GTP per 60,000 g of protein. Guanine nucleotide bound to the exchangeable site of tubulin was converted to GDP during microtubule assembly, while GTP at the non-exchangeable site remained intact. 3. Guanine nucleotide which had been bound to the exchangeable site of tubulin before microtubule assembly was also exchangeable during disassembly.

在存在鸟苷三磷酸（GTP）的情况下从猪脑中纯化得到的微管蛋白，每60,000克蛋白质含有0.16摩尔的鸟苷二磷酸（GDP）和0.73摩尔的GTP。2. 由纯化的微管蛋白重构的微管每60,000克蛋白质含有0.43摩尔的GDP和0.41摩尔的GTP。在微管组装过程中，与微管蛋白可交换位点结合的鸟嘌呤核苷酸转化为GDP，而在不可交换位点的GTP保持完整。3. 在微管组装前与微管蛋白可交换位点结合的鸟嘌呤核苷酸在拆卸过程中也是可交换的。

J Biochem. 1975 Jun;77(6):1193-7.

Tubulin purified from porcine brain in the presence of GTP contained 0.16 mole of GDP and 0.73 mole of GTP per 60,000 g of protein. 2. Microtubules reconstituted from the purified tubulin contained 0.43 mole of GDP and 0.41 mole of GTP per 60,000 g of protein. Guanine nucleotide bound to the exchangeable site of tubulin was converted to GDP during microtubule assembly, while GTP at the non-exchangeable site remained intact. 3. Guanine nucleotide which had been bound to the exchangeable site of tubulin before microtubule assembly was also exchangeable during disassembly.

在存在鸟苷三磷酸（GTP）的情况下从猪脑中纯化得到的微管蛋白，每60,000克蛋白质含有0.16摩尔的鸟苷二磷酸（GDP）和0.73摩尔的GTP。2. 由纯化的微管蛋白重构的微管每60,000克蛋白质含有0.43摩尔的GDP和0.41摩尔的GTP。在微管组装过程中，与微管蛋白可交换位点结合的鸟嘌呤核苷酸转化为GDP，而在不可交换位点的GTP保持完整。3. 在微管组装前与微管蛋白可交换位点结合的鸟嘌呤核苷酸在拆卸过程中也是可交换的。