Lipkin G, Knecht M E
Schweiz Med Wochenschr. 1975 Oct 18;105(42):1360-4.
Contact inhibition of growth is the in vitro property whose loss is most closely correlated with in vivo tumorigenicity. A contact inhibited line of hamster melanocytes produces a diffusible high molecular weight protein (melanocyte contact inhibitory factor, MCIF) which restores contact inhibition of growth to hamster malignant melanocytes. MCIF, extracted from culture medium of confluent contact-inhibited cultures, is now shown to restore the same capacity for in vitro growth regulation to malignant melanocytes of human and murine origins. In all 3 species MCIF produces characteristic changes in growth and morphology of cultures, with significant reductions of saturation densities. MCIF, the first protein identified which restores contact inhibition of growth to malignant cells, may be the prototype for a closely related class of surface-associated proteins concerned with regulation of normal cell-cell interactions leading to feedback inhibition of growth.
生长接触抑制是一种体外特性，其丧失与体内致瘤性最为密切相关。一条接触抑制的仓鼠黑素细胞系产生一种可扩散的高分子量蛋白质（黑素细胞接触抑制因子，MCIF），它能将生长接触抑制恢复到仓鼠恶性黑素细胞。从汇合的接触抑制培养物的培养基中提取的MCIF，现已证明能将相同的体外生长调节能力恢复到人和鼠源的恶性黑素细胞。在所有这三个物种中，MCIF都会使培养物的生长和形态发生特征性变化，饱和密度显著降低。MCIF是第一种被鉴定出能将生长接触抑制恢复到恶性细胞的蛋白质，它可能是一类密切相关的表面相关蛋白质的原型，这类蛋白质与正常细胞间相互作用的调节有关，从而导致生长的反馈抑制。
