Legaz M E, Weinstein M J, Heldebrant C M, Davie E W
Ann N Y Acad Sci. 1975 Jan 20;240:43-61. doi: 10.1111/j.1749-6632.1975.tb53321.x.
A new method has been described for the isolation of factor VIII. The method results in a high yield of factor VIII that is homogeneous by several different criteria. The purified protein is very stable and is not dissociated in the presence of 1 M NaCl or 0.25 M CaCl2. The highly purified protein is readily activated and inactivated by various proteolytic enzymes, such as thrombin, plasmin, and trypsin. The molecular events that lead to the activation reaction, however, have not been established.
已描述了一种分离凝血因子VIII的新方法。该方法可产生高产量的凝血因子VIII，根据几种不同标准，其具有均一性。纯化后的蛋白质非常稳定，在1M氯化钠或0.25M氯化钙存在的情况下不会解离。高度纯化的蛋白质很容易被各种蛋白水解酶激活和失活，如凝血酶、纤溶酶和胰蛋白酶。然而，导致激活反应的分子事件尚未明确。
