Mueller Xavier M, Tevaearai Hendrik T, Jegger David, Augstburger Monique, Goddar Gilles, von Segesser Ludwig K
Department of Cardiovascular Surgery, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
ASAIO J. 2002 Sep-Oct;48(5):483-6. doi: 10.1097/00002480-200209000-00008.
Trillium coating (Medtronic Inc., Minneapolis, MN) offers, in addition to the presence of heparin, endothelium-like properties of its negatively charged surface. Its thromboresistant properties on coated connectors are tested here and compared with uncoated standard connectors, as well as with the Carmeda BioActive surface (CBAS) heparin surface coating. A partial cardiopulmonary bypass bovine model (body weight 68 +/- 5 kg) was selected, and the surfaces were exposed to the blood stream (pump flow 3.5 L/min) for up to 350 minutes without systemic heparinization. Thereafter, another set of samples was exposed to stagnant blood for 20 minutes. Besides hemodynamic, hematologic, and biochemical analyses, the macroscopic appearance of 45 blood exposed surface samples were graded semiquantitatively on a scale of 0 to 10: no macroscopic deposits = grade 0, one spot (1 mm diameter) = grade 1, two spots = grade 2, five or more spots = grade 5, 10% of the surface covered with clots = grade 6, 100% covered = grade 10. When exposed to blood flow, Trillium and CBAS coatings showed a statistically significant (p = 0.03) better thromboresistance (score: 0 +/- 0 for both) than uncoated connectors (score: 0.8 +/- 1.5) in this nonheparinized model. The same holds true when the connectors were exposed to stagnant blood (score: 0 +/- 0 for both coatings vs 4.3 +/- 2.8 for controls; p = 0.03). Therefore, Trillium coating exhibits significant antithrombotic properties that outperform standards for connectors used in clinical perfusion.
除了含有肝素外，Trillium涂层（美敦力公司，明尼阿波利斯，明尼苏达州）其带负电荷的表面还具有类似内皮的特性。在此测试其在涂层连接器上的抗血栓性能，并与未涂层的标准连接器以及Carmeda生物活性表面（CBAS）肝素表面涂层进行比较。选择了部分体外循环牛模型（体重68±5千克），在未进行全身肝素化的情况下，将这些表面暴露于血流中（泵流量3.5升/分钟）长达350分钟。此后，另一组样本暴露于静态血液中20分钟。除了进行血流动力学、血液学和生化分析外，还对45个暴露于血液的表面样本的宏观外观进行了半定量分级，范围为0至10级：无宏观沉积物=0级，一个斑点（直径1毫米）=1级，两个斑点=2级，五个或更多斑点=5级，10%的表面被血栓覆盖=6级，100%覆盖=10级。在这个未肝素化的模型中，当暴露于血流时，Trillium涂层和CBAS涂层显示出比未涂层连接器具有统计学意义（p = 0.03）的更好抗血栓性（评分：两者均为0±0）（未涂层连接器评分：0.8±1.5）。当连接器暴露于静态血液时情况相同（两种涂层评分：均为0±0，而对照组为4.3±2.8；p = 0.03）。因此，Trillium涂层具有显著的抗血栓性能，优于临床灌注中使用的连接器标准。
