Lacelle Chantale, Wang Eugenia
Department of Anatomy and Cell Biology, McGill University, 3640 University Street, Que, Montreal, Canada.
Mech Ageing Dev. 2002 Jul;123(10):1415-8. doi: 10.1016/s0047-6374(02)00074-x.
In population studies involving peripheral blood samples from nonagenarian and centenarian donors, the amount of biological material available for research is restricted, as only a few millilitres of blood can be obtained from extremely old donors without significantly compromising their health. Here we describe a protocol to immortalize small amounts of total frozen blood from extremely old donors (90+) using the Epstein-Barr virus, despite the low level of circulating B cells present in nonagenarian and centenarian blood samples. This methodology provides a unique way to maximize resources of biological material available for research.
在涉及来自九旬和百岁捐赠者外周血样本的人群研究中，可用于研究的生物材料数量有限，因为从极其高龄的捐赠者身上只能获取几毫升血液，且不会对他们的健康造成显著损害。在此，我们描述了一种方案，尽管九旬和百岁老人血液样本中循环B细胞水平较低，但仍可使用爱泼斯坦-巴尔病毒使来自极其高龄捐赠者（90岁以上）的少量全冻血永生化。这种方法提供了一种独特的方式，可最大限度地利用可用于研究的生物材料资源。
