Kolb E
Arch Exp Veterinarmed. 1975;29(5):735-58.
Behaviour of the alkaline phosphatase (AP) present in blood serum, blood plasma, bile and extracts of organs (kidney, liver, intestinal mucosa, bone) was studied under various conditions of incubation (after preliminary heat treatment and after addition of L-phenyl-alanine). AP in serum and bile from calves and bullocks was very heat labile and did not inhibit phenylalanine much. EDTA and citrate were active inhibitors of AP, and therefore blood samples containing these anticoagulants are unsuitable for enzyme investigations. Out of four compounds tested, the substrate that was most rapidly split by AP from tissues was disodium phenylphosphate. AP activity was particularly high in kidney. The most heat-labile form of AP was that present in bone. AP in different tissues was inhibited to different extents by phenylalanine.
研究了血清、血浆、胆汁及器官（肾脏、肝脏、肠黏膜、骨骼）提取物中碱性磷酸酶（AP）在不同孵育条件下（初步热处理后以及添加L-苯丙氨酸后）的活性。犊牛和公牛血清及胆汁中的AP对热非常不稳定，对苯丙氨酸的抑制作用也不大。乙二胺四乙酸（EDTA）和柠檬酸盐是AP的活性抑制剂，因此含有这些抗凝剂的血样不适用于酶学研究。在测试的四种化合物中，组织中的AP最快速分解的底物是苯磷酸二钠。肾脏中的AP活性特别高。AP最不耐热的形式存在于骨骼中。不同组织中的AP受苯丙氨酸的抑制程度不同。
