Benyajati S, Morris R J
Aust J Exp Biol Med Sci. 1975 Dec;53(6):489-98. doi: 10.1038/icb.1975.54.
Sheep erythrocytes of high and low potassium types were incubated in non-haemolytic hypotonic and hypertonic media for 4-5 h at 30 degrees. After initial swelling or shrinking, they readjusted their volume toward their initial isotonic volume. The volume regulation was associated with specific changes in cation fluxes. In the swollen cells, efflux of both sodium and potassium was increased and influx of both cations was slightly decreased; the converse was true for the shrunken cells. All four fluxes were changed in a direction that led to return to normal volume. The difference in the response of the two types of sheep erythrocytes to changes of extracellular fluid osmolality resided in the different activity of their cation transport systems. It is concluded that sheep erythrocytes possess some means of regulating their volume in vitro which is linked to cation permeability. The exact nature of the physical mechanisms by which they accomplish this remains to be elucidated.
将高钾型和低钾型绵羊红细胞在30摄氏度的非溶血低渗和高渗培养基中孵育4 - 5小时。在最初的肿胀或收缩之后，它们将体积重新调整至初始等渗体积。体积调节与阳离子通量的特定变化相关。在肿胀的细胞中，钠和钾的外流均增加，两种阳离子的内流略有减少；对于收缩的细胞则情况相反。所有这四种通量的变化方向均导致细胞恢复到正常体积。两种类型的绵羊红细胞对细胞外液渗透压变化的反应差异在于其阳离子转运系统的活性不同。得出的结论是，绵羊红细胞在体外具有某种调节其体积的方式，这种方式与阳离子通透性相关。它们实现这一过程的确切物理机制仍有待阐明。
